湖泊是地球上重要的淡水资源库，洪水调蓄库和物种基因库。云南高原地质结构复杂，湖泊地质历史悠久，生物区系十分独特。浮游病毒是海洋生态系统中极其重要的一员，在调节生物群落结构、能量物质循环、基因转移和气候变化等方面具有重要作用，但关于其在湖泊特别是高原富营养化湖泊中生态功能的知识比较缺乏。在前期工作基础上本项目拟开展以下研究：利用宏基因组测序技术揭示高原富营养湖泊中浮游病毒多样性及其与环境因子相关性；分离气单胞菌和蓝细菌（蓝藻）烈性噬菌（藻）体，分析它们的生理生化和基因组特征，为开发新型噬菌（藻）体杀菌（藻）剂储备资源；通过标记基因的Q-PCR和克隆文库揭示浮游病毒在调控高原富营养化湖泊中藻类群落丰度和结构的功能。本研究将有助于深入了解浮游病毒在高原富营养化湖泊中的多样性和对藻类群落的调控，为富营养化湖泊蓝藻“水华”治理提供新思路，为气单胞菌感染的防控提供新资源，具有较好的科研价值和应用前景。

Lakes are important fresh water resource on the earth, the flood storage library and species gene pool. Geological structure is complex，lake has a long history of geology，biological flora is unique in Yunnan plateau. Virioplankton is extremely important member in marine ecosystems，play great roles in the marine ecological system such as modulating the biodiversity and species population, regulating the nutrient cycling , intervening gene transfer and influencing climate changes. But there is little knowledge about the ecological function of the virioplankton in the lakes, especially plateau lakes. On the basis of our previous work, this project intends to carry out the following study. Firstly, this project will use metagenomic technology to determine virioplankton diversity and its correlation with environmental factors in plateau eutrophic lake. Secondly, we will isolate virulent virus of Aeromonas sp. and Cyanobacteria for understanding the diversity and distribution characteristics of these two types of virus, and reserve viral resources for development of new type of antimicrobial agents. Finally, this project will use marker gene Q-PCR and clone library to determine how the cyanophage and phycodnavirus regulate Cyanobacteria and Synechococcus community abundance and structure. This study will contribute to understanding virioplankton characteristics of distribution and ecological function in plateau eutrophic lakes, providing new ideas for governance the eutrophic lakes Blooms, reserving new viral resources for prevention and control of aquatic common infection.