环境中抗生素抗性基因的高灵敏度毛细管电泳质谱及激光诱导荧光检测方法研究

抗生素抗性基因（ARGs）作为一种新型环境污染物，其保守与变异的特性研究是研究环境胁迫等因素对抗生素耐药性发生发展的重要研究内容。由于ARGs具有分布广泛、种类繁多、难以处理等特点，环境中ARGs的定性定量分析十分困难。目前的研究涵盖了ARGs在环境中的存在形式、迁移转化、定性定量和作用机制等方面，但研究方法主要基于定量效果不佳的高通量测序技术以及PCR/qPCR技术，这些方法还面临样品消耗量多、工作量大等问题。为适应更加深入的研究和治理需要，本课题拟针对ARGs开发出一套高效灵敏的分离检测流程，设计新型共价有机骨架材料、共价有机高分子材料用以提取与富集水体样本中的ARGs，并依托毛细管电泳质谱联用技术和毛细管电泳激光诱导荧光法进行定性定量研究和多重检测，且在此基础上研究表观遗传修饰与胞内胞外ARGs作用机制的关系，以期补充或发现新的ARGs作用机制，为ARGs消除方法的开发提供新的思路。

Antibiotic resistance gene (ARGs) as a new type of environmental pollutants has attracted more and more attention due to its wide distribution, wide varieties and difficulties in treatment. Current researches cover the existence forms, migration and transformation, qualitative and quantitative analysis and action mechanism of ARGs in the environment, etc., but the research methods are mainly based on high-throughput sequencing technology and PCR/qPCR technology, which face the problems of poor quantitative ability, large sample comsumption and huge workload. To meet the need of more in-depth research and pollution control, we intend to establish a highly efficient and sensitive procedure for separation and detection of ARGs in this study. We will develop new covalent organic framework or covalent organic polymer materials for extraction and enrichment of ARGs in water samples and apply sheathless interfaced capillary electrophoresis - mass spectrometry and laser induced fluorescence for quantification research and multiple detection. Upon these, we plan to explore the relationship between epigenetic modifications and ARGs functioning mechanism, in order to supplement or even discover new mechanisms and provide new ideas for the development of ARGs elimination methods in the environment.