基于毛细管电泳的蛋白质糖基化谱高灵敏度方法研究

多糖是自然界含量最丰富的生物聚合物, 广泛表达于微生物、植物和动物中, 并以糖基化的方式形成糖蛋白参与所有蛋白质分子的结构稳态和功能调节，主要包括细胞信号传递、基因表达、免疫响应以及蛋白质折叠等方面，蛋白质糖基化的变异是生物体发生变异的主要途径之一，准确高灵敏度测定蛋白质糖基化变化是理解和调节生命行为的基础工作。本课题拟以建立蛋白质糖基化特征谱高灵敏度分析方法为目标，通过新型免疫识别体系-新衍生方法-同位素标记衍生试剂相结合，开发出基于毛细管电泳-激光诱导荧光高灵敏度检测及毛细管电泳-无鞘液接口质谱鉴定的糖基化变异检测的新方法，为蛋白质糖基化的快速鉴定特别是疾病前期预警建立有效工具。

Carbohydrate is one of the most important and high abundence biomacromolecules in nature. The characterization and the development of glycomics in recent years has been driven by an increased understanding of the many different roles that carbohydrates play in biology. Carbohydrates attached to proteins, known as glycans, are important determinants in cell signaling, gene expression, immune response, protein function, and protein folding. Glycans profiling is one of primary tool to find the post transferation expresion in life process. This project is focused on the development of high sensitive tools for glycans profiling based on capillary electrophoresis including design a novel derivative reagent system. With the novel enrichment and sensitive labelling reagent, the discrimation in glycan profiling can be demonstrated sensitivily.