云南重楼中重楼皂苷类物质积累的分子机制研究

云南重楼（Paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz.）是传统名贵中药材重楼的重要来源，重楼原料主要依赖人工栽培，栽培年限长。项目组研究发现不同来源的云南重楼指标成分重楼皂苷的含量差异较大，严重制约重楼的原材料供应和质量保证。本项目拟以不同来源的云南重楼为研究对象，比较云南重楼、七叶一枝花（Paris polyphylla var. chinensis (Franch.) Hand）和长柱重楼（Paris forrestii (Takht.) H. Li）根茎的转录组变化，挖掘参与重楼皂苷生物合成的OSC、P450s和UGTs中的关键酶基因，进行功能分析，建立基因表达、酶活性与重楼皂苷类物质成分的关系，明确调控重楼皂苷的关键基因，解析这些关键基因在重楼皂苷积累中的分子机制，为云南重楼的栽培与开发利用提供理论基础。

Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz. (P. yunnanensis) is an important source of valuable traditional Chinese medicines Paridis Rhizoma, which relies mainly on artificial cultivation with a long cultivation period. However, the study of the project team found that the contents of Rhizoma Paridis saponins from different sources differed greatly, which had seriously restricted the supply and quality assurance of raw materials of P. yunnanensis. In this study, P. yunnanensis from different habitats would be selected as the experimental materials to compare the transcriptome changes of rhizomes with Paris polyphylla var. chinensis (Franch.) Hand and Paris forrestii (Takht.) H. Li. At the same time, oxidosqualene cyclase (OSC), cytochrome P450 monooxygenases (P450s) and UDP-dependent glycosyltransferases (UGTs) genes involved in the biosynthesis of saponins were mined and the functional analysis was carried out to establish the relationship between gene expression, enzyme activity and Rhizoma Paridis saponins. The key genes regulating the Rhizoma Paridis saponins and the molecular mechanism of these key genes in the accumulation of saponins were analyzed, so as to provide a theoretical basis for the further cultivation, development and utilization of P. yunnanensis.