活性氧物质（ROS）调控滇重楼种子萌发成苗的分子机制研究

滇重楼（Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz.）是传统名贵中药材重楼的重要来源，是国家重点保护野生濒危物种。中药材重楼原料主要依赖人工栽培，但是滇重楼种子休眠期、成苗期长，严重限制滇重楼人工栽培。因此，阐明滇重楼种子萌发成苗的分子机制，对破除休眠、促进快速成苗具有重要意义。本项目拟在前期研究基础上，选择最佳滇重楼种子处理方法，比较萌发与未萌发、成苗与未成苗种子中转录组变化，鉴定调控活性氧物质（ROS）产生与清除的相关基因；在不同萌发阶段动态分析ROS相关生理生化、基因表达变化，确定ROS调控关键基因；结合转录组数据，分析ROS调控网络，关键基因功能验证；同时探讨ROS与激素的互作关系，初步阐明滇重楼种子形态和生理休眠的分子差异，解析ROS调控滇重楼种子萌发成苗的分子机制，为滇重楼栽培与资源保护提供理论依据。

Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz. is an important source of Chonglou (PARIDIS RHIZOMA), which is a valuable traditional Chinese medicine, and it is a wild and endangered species under the state's protection. Chonglou relies mainly on artificial cultivation. However, the long seed dormancy period and seedling stage have severely limited the artificial cultivation of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz.. Therefore, elucidating the molecular mechanism of seed germination and seedling formation is of great significance to breaking dormancy and promoting rapid seedling establishment of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz.. The best seed treatment method would be selected based on the previous studies. The genes involved in the production and elimination of reactive oxygen species (ROS) would be identified by comparing the transcriptome among germinating and non-germinating, seedling and non-seedling seedlings. At different stages of germination, ROS-related physiological, biochemical and gene expression were dynamically analyzed to determine ROS-regulated key genes. Combined with the transcriptome data, the ROS regulatory network was analyzed to validate the key genes. At the same time, the interaction between ROS and hormones was explored, and the molecular differences of seed morphological and physiological dormancy were analyzed. The molecular mechanism of ROS regulating seed germination and seedling formation provides the theoretical basis for the future cultivation and resource conservation of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz..