L-半胱氨酸修饰的纳米酶催化降解中药中的马兜铃酸I和II及其快速检测方法的研究

被列为1类致癌物的马兜铃酸I和II，广泛存在于马兜铃属和细辛属等植物和药材中。加强对中药中的马兜铃酸I和II监控和分析，对保障人们身体健康和充分利用我国中药资源具有重要意义。研究表明，L-半胱氨酸能降解马兜铃酸I和II，但存在降解速度慢、效率低的突出问题。纳米酶具有良好的催化性能，将L-半胱氨酸修饰的纳米酶应用于马兜铃酸I和II降解，有望提高其降解速度和降解效率，并实现对其快速检测。本项目拟制备出能快速催化降解马兜铃酸I和II为相应马兜铃次酸的L-半胱氨酸修饰的纳米酶，并对纳米酶催化降解条件进行优化，阐明纳米酶的催化性能与催化降解机制；然后分别制备出能快速检测马兜铃次酸I和II的单克隆抗体型和核酸适配体型的免疫层析试纸条，应用于中药中的马兜铃酸I和II快速检测。项目的研究成果将为中药中其它有害成分的降解提供经验借鉴，为加强中药中马兜铃酸的监控提供技术支持，具有广泛的应用价值和市场前景。

Aristolochic acid I and II, which are classified as 1 types of carcinogens, are widely found in Aristolochic and Asarum and other plants and herbs. Strengthening the monitoring and analysis of aristolochic acid I and II in traditional Chinese medicine is of great significance for ensuring people's health and making full use of Chinese traditional medicine resources. Studies have shown that L-cysteine can degrade aristolochic acid I and II, but there are outstanding problems of slow degradation and low efficiency. Nanozyme has good catalytic performance. The application of L-cysteine modified nanozyme in the degradation of aristolochic acid I and II will expect to improve the degradation efficiency and realize their rapid indirect detection. In this project, the L-cysteine modified nanozyme, which can rapidly catalyze degradation of aristolochic acid I and II, will be prepared, and the preparation and catalytic conditions of nanozyme will be optimized. The catalytic properties and degradation mechanism of nanozyme will be elucidate. Then, the monoclonal antibody and aptamer type of immunochromatography test strips, which will be used for individual and simultaneous indirect detection of aristolochic acid I and II in traditional Chinese medicine, will be prepared,.respectively. The research results will provide experience and reference for the degradation of other harmful components in traditional Chinese medicine, and provide technical support for strengthening the monitoring of aristolochic acid. It has broad application value and market development prospects.