真菌毒素是中药中存在的一类典型的有害物质，加强对中药中的真菌毒素监控和分析，是中药质量安全评价的重要内容之一。建立快速、简单和可靠的同时对中药中多种真菌毒素的筛查和检测的免疫层析技术显得越来越重要。本项目拟通过将抗体的Fab片段定向固定后，再与金纳米花颗粒结合，优化各项工艺条件，制备出同时检测两种和三种真菌毒素的高灵敏度免疫层析试纸条。同时对试纸条的灵敏度、稳定性、特异性和重复性等性能进行评价，并开展其在中药中真菌毒素检测中的应用评价研究。项目的研究成果可应用于卫生监督、药厂、医院和家庭等现场快速初筛检测，从而为有效预防和控制中药中真菌毒素污染提供技术支撑，具有良好的社会和经济价值。

Mycotoxins are a class of typical harmful substances in traditional Chinese medicine. The mycotoxins are monitored and analyzed in traditional Chinese medicine, and this is one of the important contents for the quality and safety of traditional Chinese medicine. To establish a simple, rapid and reliable immunechromatography technology for screening and detection of multiple mycotoxins in traditional Chinese medicine is becoming more and more important. In this study, the immunochromatographic strips of simultaneous detection for two or three kinds of mycotoxins will be prepared by the connection with oriented immobilization of antibody Fab fragment and urchin-like gold nanoparticle. The preparation conditions will be optimized. The sensitivity, stability, specificity and repeatability of the test strip will be evaluated, and the evaluation method will be developed by using the test strip for detection mycotoxins in traditional Chinese medicine. The immunochromatographic strips will be used for rapid screening detection of mycotoxins in traditional Chinese medicine for the health supervision, pharmaceutical companies, hospitals and family. The results of the project will be provide technical support for effective prevention and control of mycotoxin contamination in the traditional Chinese medicine, and have good social and economic value.