Bt毒素Cry51Aa在绿盲蝽中肠的结合蛋白分析及功能受体鉴定

研发可用于防治半翅目刺吸式害虫的生物农药一直是生产中迫切需要解决的问题，一些新型Bt毒素如Cry51Aa等的发现填补了这方面的空白。与经典三结构域型Cry毒素不同，Cry51Aa属于Aerolysin型β-穿孔蛋白，对半翅目昆虫盲蝽具有杀虫活性。目前对Bt毒素作用机制的研究主要集中于Cry1类毒素和鳞翅目昆虫，对半翅目昆虫的毒杀机制研究几乎没有。本项目拟综合利用多种蛋白互作分析技术及基因功能鉴定手段，检测Cry51Aa毒素与绿盲蝽中肠细胞的结合过程、筛选毒素在中肠上的结合蛋白、鉴定毒素结合蛋白的受体功能，从而解析Cry51Aa毒素对绿盲蝽产生毒性的关键步骤。研究结果将丰富并完善Bt毒素的作用机制，同时为开展刺吸式昆虫对Cry51Aa毒素的抗性机理研究奠定基础。

It has been an urgent issue in plant protection to develop biological pesticides for the control of hemiptera sap-sucking pests. The discovery of some new Bt toxins, such as Cry51Aa, has filled the gap in this field. Unlike the classical 3-domain Cry toxins, Cry51Aa is an aerolysin-type β-pore-forming toxin and has insecticidal activity to mirids. Most of the studies on mode of action of Bt toxins are focusing on Cry1A and Cry2A insecticidal proteins against lepidopteran insects, whereas studies on the mechanism of action of Cry51Aa to hemipteran insects are very limited. This project aims to identify the key factor of mechanism of action of Cry51Aa toxin to Apolygus lucorum by analyzing the binding process between Cry51Aa toxin and the midgut cells of A. lucorum, screening the binding proteins to the toxin in the midgut cells, and finally identifying the functional receptors of Cry51Aa. The results are expected to improve our understanding of the mode of action of Bt toxin Cry51Aa, lay a foundation for further research on the resistance mechanism to Cry51Aa in hemiptera insects.