海洋沉积物核心菌群Woeseiaceae/JTB255的分离、分类系统构建及其环境适应机制研究

伍斯氏菌（Woeseiaceae/JTB255）类群属于变形细菌门γ变形菌纲，其丰度高、分布广泛，是全球海洋沉积物微生物区系中的核心类群。2016年本课题组报道了此类群第一个可培养菌株海洋伍斯氏菌（Woeseia oceani）XK5T，并建立了伍斯氏菌科。本课题计划在前期工作基础上，进一步优化伍斯氏菌的分离培养方法，分离得到10株左右伍斯氏菌；完成新种属的多相分类与伍斯氏菌分类系统构建；通过基因组学和比较基因组学分析伍斯氏菌的进化关系和代谢特征，检测不同培养条件下伍斯氏菌的代谢能力，结合差异转录组学分析伍斯氏菌的代谢途径转换方式，通过异源表达验证碳氮硫关键代谢基因的功能，结合理论分析及实验模型解释该类群在海洋物质循环中的作用及其环境适应机制。

Woeseiaceae/JTB255, belongs to the class gammaproteobacteria, phylum Proteobacteria, cosmopolitan and abundant core members of microbial communities in marine sediments. The first cultured strain Woeseia oceani XK5T was reported by our group in 2016, and Woeseiaceae was established at the same time. In this study, we will further optimize the isolation technique for woeseibacteria to obtain about 10 strains. Polyphasic taxonomy of the new strains will be carried out to construction the classification system of woeseibacteria, and many new taxa will be proposed. The evolutionary relationships and metabolic characteristics of woeseibacteria will be studied by genome and comparative genomics analysis. To analyze the changes of metabolism patterns of woeseibacteria, the metabolic levels and the changes of gene transcripts will be examined under different culture conditions. Validation of the function of genes related to carbon, nitrogen and sulfur metabolism will also be carried out by heterologous expression. Combined with theoretical analysis and experimental model, the roles of this group in marine material cycle and its environmental adaptability will be clarified.