牛至精油缓解早期断奶犊牛氧化应激作用及机理研究

早期断奶引发氧化应激是诱发“断奶应激综合症”的重要原因，Nrf2/AREt是维持细胞内氧化还原平衡、抗氧化应激的重要通路，植物精油中活性成分激活Nrf2依赖的适应性应答是其提高机体抗氧化能力的有效调控手段。牛至精油是由牛至提取的植物精油，抗氧化潜力为96.6-137.5mmol。然而，关于牛至精油缓解早期断奶犊牛氧化应激作用机理尚不明确，关于牛至精油调控Nrf2/ARE通路缓解早期断奶犊牛肠道氧化损伤的研究未见报道。本研究首先以早期断奶犊牛体内氧化应激模型为研究对象，初步探索牛至精油缓解早期断奶犊牛氧化应激的作用和机理；紧接着以早期断奶犊牛体外模型为研究对象，通过isRNA干扰技术挖掘牛至精油对缓解早期断奶犊牛氧化应激的分子调控机制，探究牛至精油可否通过调控Nrf2/ARE通路缓解氧化应激；最后，通过体内和体外模型试验结果的相互验证，进一步揭示牛至精油缓解早期断奶犊牛氧化应激的调控机制。

Oxidative stress caused by early weaning is an important cause of “weaning stress syndrome”. Nrf2/ARE signaling pathway is a system that converts exogenous stimuli into eukaryotic transcriptional response, and is an important pathway to maintain intracellular REDOX balance and antioxidant stress. Active ingredients in essential oils activate Nrf2-dependent adaptive responses, which is considered to be an effective regulatory means for plant extracts to improve the body's antioxidant capacity. Oregano essential oil is a plant essential oil extracted from oregano with antioxidant potential of 96.6-137.5mmol. However, the mechanism of oregano oil to relieve oxidative stress in early weaned calves is still unclear and studies on whether oregano oil can alleviate intestinal oxidative damage in early weaning calves by regulating the Nrf2/ARE signaling pathway have not been reported. In this study, the in vivo model of early weaned calves was firstly used as the research object to preliminarily explore the effect and mechanism of oregano essential oil to relieve the oxidative stress of early weaned calves. Secondly, the in vitro model of early weaning calf was taken as the research object, and the molecular regulation mechanism of oregano essential oil on relieving oxidative stress of early weaning calf was explored by using isRNA interference technology, to explore whether oregano essential oil could alleviate oxidative stress by regulating Nrf2/ARE signaling pathway. Lastly, the regulation mechanism of oregano essential oil to relieve oxidative stress in early weaned calves was further revealed through the mutual verification of in vivo and in vitro model test results.