拟南芥转录因子IORG5/6通过调控BIK1表达参与植物免疫系统建成的机制研究

成熟完善的免疫系统在植物抗病菌反应中是至关重要的，但关于免疫系统在植物发育早期是如何建成的机制研究甚少。前期研究中，我们鉴定到两个响应flg22的同源锌指类转录因子IORG5/6（Immunity Ontogeny-Regulated Gene 5/6）。基因表达、生化和表型分析初步显示，IORG5/6负调控植物早期发育阶段免疫反应，而且可能通过抑制BIK1基因的表达发挥功能。在本项目中，我们以模式植物拟南芥为实验材料，开展以下研究：1）深入分析IORG5/6在早期发育阶段免疫信号通路中的功能；2）进一步解析IORG5/6对BIK的调控作用；3）系统阐明IORG5/6如何通过转录水平调控BIK1基因的表达，控制植物早期发育阶段免疫系统建成的分子机理，以期为作物的抗病育种，尤其是幼苗抗病能力改良提供理论依据。

Plants immune system was very important for plant disease resistant, but little is known about the mechanism of how the immune system is built in the early stages after germination. In the previous study, we identified two homologous zinc finger transcription factors, IORG5/6 (Immune Ontogeny Regulated Gene 5/6), whose expression patterns were opposite in the postgermination stage, but both of them could inhibit the expression of BIK1. Further analysis showed that IORG5/6 may negatively regulate plant immunity in the postgermination stage. Based on these former results, in this work, we will work through the following aspects: 1) To analyze the function of IORG5/6 in plant innate immunity in early stage after germination; 2) To confirm the regulation of BIK1 by IORG5/6; 3) Reveal the molecular mechanism of IORG5/6 regulating plant innate immune response through BIK1 gene during the postgermination stage, so to provide new ideas and genetic resources for disease-resistant plants breeding, especially for the improvement of seedlings disease resistance ability.