病害是造成农作物产量降低、品质下降的重要因素之一。植物免疫信号通路中起关键作用的PRR的研究，对阐明抗病分子机理、培育抗病品种具有重要意义。前期研究中，我们鉴定到一个FLS2的转录抑制因子SOF1（Supressor Of FLS2 1），并发现该基因受miRNA（miR_PIR1）的调控。初步研究发现，35S::miR_PIR植株及sof1突变体在flg22处理后生长抑制加强，sof1突变体MAPK级联反应增强，表明miR_PIR1-SOF1可能通过FLS2基因参与对植物天然免疫的负调控作用。在本项目中，我们拟以拟南芥为实验材料，从三个方面开展研究工作：1）深入分析SOF1基因在免疫信号通路中的功能；2）进一步验证miR_PIR1对SOF1基因的调控作用；3）系统阐明miR_PIR1- SOF1通过FLS2基因参与免疫信号通路的负调控的分子机理，以期为作物的抗病育种提供新思路和基因资源。

The plant disease is one of the most important factors affecting crop yields and quality. Therefor，it has a very important significance to understand the molecular mechanisms of PRR in plant disease resistance. In the previous studies, we found a transriptional supressor of FLS2, SOF1 gene (Supressor Of FLS2 1), and SOF1 may be regulated by miRNA (miR_PIR1). The non-function mutant sof1 was hypersensitive to flg22, and showed enhanced MAPK activity. These results illustrated SOF1 may regulate PTI signaling by supressing the expressing of FLS2. Based on these former results, in this work,we will work through the following aspects: 1) To analyze the function of SOF1 in plant resistance to pathogen response; 2)To confirm the regulation of miR_PIR1 and SOF1 gene; 2) Reveal the molecular mechanism of miR_PIR1- SOF1 regulating plant innate immune response through FLS2 gene, so to provide new ideas and genetic resources for disease-resistant plants breeding.