亚毫米尺寸植入式多通道微磁刺激装置设计及其对海马区Schaffer-CA1突触可塑性靶向性协同调控机制的研究

亚毫米尺寸多通道mMS作为一种新型的磁刺激技术，具有体积小、靶向性强和多点协同调控等优点，但其未在深部海马合团实现植入式应用，对学习和记忆的作用机制尚不清楚。本项目首先确定离体多通道mMS对海马PP-DG-CA3-CA1神经通路调控的最优靶点和磁刺激参数，结合有限元分析方法，设计线圈参数和阵列结构，解决其生物相容性、多路驱动控制、实验安全性等关键问题，通过研究不同阵列模板提高感应电场聚焦性，降低线圈功率耗散，实现一款活体植入式多通道mMS装置。然后研究其在活体大鼠海马神经合团的植入方法，解决携带可靠性和舒适性等问题，设计长时程磁刺激协议，研究多通道mMS单点和多点协同刺激对突触可塑性的调控规律。采用ICP-MS技术研究磁刺激对海马脑片离子含量变化的影响规律，揭示mT量级磁场对NMDA受体依赖的Schaffer-CA1突触可塑性作用机制。本项目对植入式多通道mMS装置未来应用具有重要意义。

As a new type of magnetic stimulation technology, the sub-millimeter-sized multi-channel micro-magnetic stimulation (mMS) has the advantages of small size, strong targeting, and multi-point coordinated regulation. Since it hasn’t been implanted in the deep hippocampus, the mechanisms of action on learning and memory affected by mMS are unclear. Firstly, this project determines the optimal target and magnetic stimulation parameters of in vitro multichannel mMS in regulating hippocampal PP-DG-CA3-CA1 neural pathway, and combines the finite element analysis method to design the coil parameters and array structure which could solve the key issues of biocompatibility, multi-channel drive control, and experimental security etc. Study on different array templates to implement a living implantable multi-channel mMS by improving the focus of induced electric field and reducing the coil power dissipation. Secondly, study on the method of implanting neural complexes in living mouse to solve the problems of carrying reliability and comfort, and to design a long-term magnetic stimulation protocol to study on the regulation of synaptic plasticity by multi-channel mMS single-point and multi-point coordinated stimulation. Finally, study on the effect of magnetic stimulation on changes of key ions such as calcium in hippocampal brain slices by ICP-MS technology, and to reveal the mechanism of mT magnitude magnetic field on NMDA receptor-dependent Schaffer-CA1 synaptic plasticity. This project is of great significance for the future application of implantable multi-channel mMS devices.