亚毫米尺寸阵列式微磁刺激装置设计及其对海马区Schaffer-CA1突触可塑性调控机理的研究

微磁刺激作为一种新的神经调控技术，具有体积小、靶向性强和无创等优点使其成为未来临床的一种优先选择，然而，其对学习和记忆的作用机制尚不清楚。项目根据海马区神经通路的结构，采用有限元分析方法，从线圈产生的磁场和感应电场大小和分布角度研究磁场效应机制，设计和优化电感线圈参数和阵列结构，解决该装置的生物相容性和安全性等关键问题，并设计一款亚毫米尺寸阵列式微磁刺激装置，其具有体积小、多点协同刺激、控制灵活、靶向性强、磁刺激和微电极阵列电生理记录同步完成等优点。在此基础上，单点磁刺激海马区Schaffer侧枝神经通路，多点磁刺激PP-DG-CA3-CA1神经通路，共同探究其对海马区Schaffer-CA1突触可塑性的调控规律，研究最优磁刺激参数和最佳靶点位置，为植入式微磁装置靶向性治疗提供基础。项目将推动磁刺激技术进一步发展和更大规模应用，提高小到足以植入大脑组织的亚毫米尺寸微磁刺激线圈应用的可能性。

As a new neuromodulation technology, there are a wide range of advantages in micro-magnetic stimulation(mMS), such as small sizes, strong targeting features and non-invasiveness, which make it quite promising in future clinical research and applications. However, the underlying mechanism of the effect of mMS on learning and memory is not yet clearly elucidated. According to the structure of neural pathway in hippocampus, finite element analysis will be adopted to study the analytical mechanism of bio-magnetic effects induced by the magnetic field produced by the coil, particularly focused on the evaluation of the intensity and distribution of the coil magnetic and induced electric field. Appropriate design will be utilized to optimize the parameters of inductance coils and array structure to help eliminate or minimize key problems, such as biocompatibility and safety of the device. Furthermore, a sub-millimeter array device of mMS will be designed, which can achieve better features like multi-point synergistic stimulation, flexible control, strong targeting although with a smaller size, and can also record physiological data of neurons and the applied magnetic field during magnetic stimulation simultaneously. In our study, single point magnetic stimulation will be adopted for Schaffer collateral nerve pathway in the hippocampus and multiple magnetic stimulation will be performed on the PP-DG-CA3-CA1 neural pathways, which hopefully can research the possible regulating mechanisms of mMS on hippocampal Schaffer-CA1 synaptic plasticity, and can help explore the optimal mMS parameters and the best target position at the same time. Moreover, the research outcome may also provide the theoretical and practical basis for the targeted therapy of the implanting micro-magnetic devices. The project will promote further development and more large-scale application of magnetic stimulation technology. Additionally, the research will contribute to development of implanted devices, particularly to that of sub-millimeter-sized mMS coils into brain tissues.