甜瓜CmERECTA基因调控短蔓形成的分子机理研究

短蔓甜瓜适宜密植，能有效提高土地利用率和产量，而且能节约劳动力，降低生产成本，适应未来轻简化栽培的需求。解析甜瓜短蔓形成的遗传调控网络与分子机理是选育短蔓品种的基础和前提，但目前甜瓜上鉴定的短蔓基因较少，短蔓相关基因克隆进展缓慢，迄今有关甜瓜短蔓形成的分子机制尚不清楚。我们前期通过图位克隆的方法在甜瓜上分离到一个短蔓基因，它编码一个ERECTA类蛋白激酶。本项目拟通过过表达和基因敲除等手段进一步验证CmERECTA基因的功能，然后比较野生型材料和其Cmerecta基因近等基因系在激素、转录组和蛋白质组水平上的差异，从中挖掘甜瓜短蔓形成调控网络中的关键因子；通过酵母单杂交鉴定CmERECTA基因的上游调控因子和酵母双杂交鉴定CmERECTA互作蛋白，最终揭示CmERECTA基因调控甜瓜短蔓形成的作用机制。

The dwarf melon plants are suitable for dense planting, which can effectively increase density and land utilization as well as reduce labor cost, and meet the needs for simplified cultivation in the future. Therefore, the analysis of the genetic regulatory network and molecular mechanism of dwarf vine formation in melon is the basis and prerequisite for plant breeding. However, there are only a few dwarf genes identified in melon, and none of them has been cloned. So far, the molecular mechanism of dwarf vine formation in melon is still unclear. We have isolated a dwarf gene in melon using map-based cloning strategy, and it encodes an ERECTA protein kinase. The expression of CmERECTA in different organs is significantly reduced in the dwarf line M406. Based on our previous work, we plan to verify the function of the CmERECTA gene by over-expression and knockout of CmERECTA in melon. Then we will identify the key genes in the regulation network by comparing the transcriptome profile, proteome profile and endogenous hormones between normal melon line and its near-isogenic line of Cmerecta gene. Furthermore, we will identify the upstream regulatory factors of the CmERECTA gene by yeast one-hybrid screening and the interacted proteins by yeast two-hybrid library to finally reveal the molecular mechanism of CmERECTA in regulating dwarf formation in melon.