甜瓜Golden2-Like转录因子调节叶绿体发育的分子机理研究

叶绿体是植物进行光合作用的关键场所，而光合作用产物是作物产量和品质形成的物质基础。研究发现Golden2-Lik（GLK）转录因子在叶绿体发育中起着非常重要的作用，它可通过调节果实叶绿体的发育来提高果实的品质和风味。然而，GLK转录因子调节叶绿体发育的作用机理与调控网络尚不清楚，仍有待深入研究。课题组前期发现一个甜瓜叶色黄绿材料，其叶绿素含量、叶绿体数目、光合效率及果实品质均显著降低。我们已经图位克隆到控制该黄绿叶色性状的基因（ygl），它编码一个GLK转录因子。本项目将通过遗传转化和构建cmglk基因的近等基因系，解析该基因在甜瓜叶绿体发育中的功能及其对果实品质的影响，同时通过RNA-Seq、ChIP-Seq和酵母双杂交等手段揭示CmGLK基因调节甜瓜叶绿体发育的分子机理与调控网络。研究结果不仅能丰富和完善GLK转录因子调节叶绿体形成的分子机制，也将为甜瓜果实品质改良提供新的思路和方向。

Chloroplasts are the photosynthetic organelle in plants, and the product of photosynthesis is the material basis for crop yield and quality. Recent studies have revealed that Golden2-Like (GLK) transcription factors play important roles in chloroplast development, which can also improve fruit quality and flavor by regulating the development of fruit chloroplast. However, the molecular mechanism of GLK transcription factors in regulating chloroplast development and its regulatory networks are still not clear and remain elusive. Our research group has identified a yellow-green leaf accession in our melon germplasm collection, and the chlorophyll content and the number of chloroplasts were significantly decreased in this accession which resulted in the reduced photosynthetic efficiency and fruit quality. The yellow-green leaf phenotype was controlled by a single recession gene (named ygl), we have cloned this gene using a map-based cloning strategy，and it encoded a GLK transcription factor. In this project, we will explore the function of CmGLK gene in regulating chloroplast development and fruit quality by developing near iso-genic lines (NILs) and genetic transformation. In addition, we will combine RNA-Seq, ChIP-Seq and yeast two-hybrid to reveal the molecular mechanism and regulation network of CmGLK gene in regulating chloroplast development. The results will not only enrich the molecular mechanism of GLK transcription factor in regulating chloroplast development in higher plants, but also provide new insight for the genetic improvement of fruit quality in melon.