复杂基质样品中超痕量纳米颗粒的在线分离富集装置研发及SP-ICP-MS分析方法建立

金属元素广泛存在于大气、土壤和水体等环境介质中，其环境健康影响受到广大学者和公众的关注。近年来，越来越多的研究发现，除了常规的金属离子态，金属颗粒物在环境中广泛存在且具有很高的活性，在金属的环境行为、迁移转化、生物效应和毒性效应等过程中发挥了非常重要的作用。目前仍缺乏环境和生物体等复杂基质样品中超痕量金属颗粒物的分析方法。本项目将针对实际环境和生物体中复杂、多尺寸、超痕量的金属形态，以3D打印技术和膜过滤技术为基础，拟设计和研发金属纳米颗粒的在线分离和富集装置；利用上述装置与SP-ICP-MS方法联用，开发复杂基质样品中超痕量金属纳米颗粒的分离、鉴定、表征和定量的分析方法。利用上述分析方法初步探究典型金属在生物体内的形态分布和迁移转化规律，为后续金属元素的生物功能、环境行为和毒理效应等进一步的研究提供方法学基础。

Metals exist in various environmental medium as different species and its potential health effects has received public concerns. Recently, it has been found that except for the ionic species, the metal nanoparticles played very important roles in the behavior, transformation, biological function and toxicity effects of metals. However, there is still a lack of the method for analysis of multi-sized metal species in complex samples. Herein, a pretreatment device for on-line separation and separation of ultratrace metal nanoparticles in complex environmental and biological samples will be developed based on the 3D printing and membrane filtration techniques. By combining the pretreatment device with single particle ICP-MS technique, the analytical method for separation, identification, characterization and quantification of ultratrace nanoparticles in complex medium will be developed. These methods will be applied to investigate the speciation, transformation and potential mechanisms of typical metals (such as Hg and Pb) in bacteria. This project will provide a powerful tool and analytical platform for further investigation on the mechanisms of biological function and toxicity effects of metals.