纳米科技的快速发展和纳米材料广泛应用对环境和人体健康产生了重要的影响，日益受到人们的关注。目前还缺乏纳米材料的分析表征手段和相应的标准方法及标准品，因此发展复杂基质样品中痕量纳米材料的表征和定量方法是研究其环境行为和生物效应的重要基础。色谱分离与检测器的联用技术已成为纳米材料表征分析的重要方法之一。本申请项目拟结合毛细管电泳（CE）与单颗粒电感耦合等离子体质谱（SP-ICP-MS）的优势，设计简单高效的接口，搭建CE-SP-ICP-MS在线联用技术，发展可同时分离、鉴定、表征和定量不同表面电荷、粒径的金属或金属氧化物纳米颗粒和金属离子的分析方法。利用该联用系统对水样、生物样品和纳米消费品中的纳米材料进行表征分析，建立基于CE-SP-ICP-MS的复杂基质中金属或金属氧化物纳米颗粒的多维表征分析平台，为金属纳米材料的表征定量、环境行为和毒理学的相关研究提供高效、灵敏、可靠的方法学基础。

With the rapid development of nanotechnologies and extensive application of nanomaterial in recent decades, new concerns for the potential adverse effects of nanoparticles on environment and human health have been raised. However, a large obstacle is the lack of proper methods for characterizing and detecting of nanoparticles (NPs) in complex media and the relative standard reference material. Therefore, the development of sensitive method for characterization and analysis of NPs in complex medium is very important. Currently, the hyphenated technique of chromatographic separation and detector has become available for the characterization of NPs. In this project, we are going to establish on-line hyphenated system by combining the capillary electrophoresis (CE) with the single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) and develop sensitive analysis methods for simultaneous separation, identification, characterization and quantification of metal (or metal oxide) NPs and metal ions. By the improvement of the interface and optimization of the separation and detection conditions, the established system are going to applied for the characterization and analysis of NPs in the real water samples, biological samples and various consumer products to develop multi-dimensional characterization and analysis platform for metal (or metal oxide) NPs in complex media. The established system and developed method would provide a powerful tool for investigation the environmental fate and toxicology of NPs and rapid screening of NP-containing products.