南方水稻黑条矮缩病毒致瘤过程与分子机制研究

植物呼肠孤病毒致瘤的现象受到关注已超过七十载，然而其致瘤机制至今仍是个未解之谜。南方水稻黑条矮缩病毒（SRBSDV）隶属呼肠孤病毒科斐济病毒属，是近年来我国学者鉴定的一个威胁水稻生产的病毒新种。我们前期研究显示SRBSDV诱导的瘤在细胞类型、细胞间通道、显微结构等方面呈现出独特性，表明SRBSDV可能采取一种新的致瘤机制。因此，我们联合浙江大学一起以SRBSDV-水稻系统为模式并综合采用一系列分子生物学和显微影像学技术，如多组学、遗传转化、蛋白质互作、X射线显微镜（XRM）、块样品序列面扫描电镜（SBF-SEM）、聚焦离子束扫描电镜（FIB-SEM）等，来进一步分析鉴定瘤的形成过程、致瘤因子、致瘤机制，从而拓展植物细胞学、植物病理学知识，为发展该类病毒病防控新策略提供科学依据。

The phenomena of plant reovirus-induced tumors have been concerned for more than seventy years. However, the mechanism has been a mystery until now. Southern rice black-streaked dwarf virus (SRBSDV) is a new member of genus Fijivirus within family Reoviridae characterized by Chinese scholars and threatening rice production in recent years. Our previous studies showed that SRBSDV-induced tumors on rice stem were unique in cell types, intercellular gateways, and microscopic structures, suggesting that SRBSDV could use a novel mechanism underlying the virus-induced tumors in rice plant. Thus the SRBSDV-rice system will be used as a model in this project cooperated by Zhejiang University and our lab, in which the process of tumor development, tumorigenic factor(s) and mechanism will be further characterized by a series of molecular biotechnology and microscopic imaging, such as mult-omics, genetic transformation, protein-protein interaction, X ray microscopy (XRM), serial block face-scan electron microscopy (SBF-SEM), and focus ion bean-scan electron microscopy (FIB-SEM). This study could be contributed to extend the knowledge of plant cell and plant pathology and provide scientific basis for developing the new strategy to control the viral diseases.