鲭鱼关键产胺菌Klebsiella oxytoca关联的生物胺形成与积累机制研究

如何有效控制生物胺是鲭鱼等易腐水产食品产业能否可持续发展的关键。研究表明，微生物在生物胺形成和积累过程中起着重要作用。进一步深入探究发现K. oxytoca是鲭鱼关键产胺菌，且产胺能力与环二鸟苷酸（c-di-GMP）信号强度呈显著正相关。鉴于此，拟在前期研究基础上，通过剖析K. oxytoca氨基酸脱羧酶基因簇组成及其在生物胺形成中的作用，揭示基于c-di-GMP信号代谢通路的生物胺形成与积累机制，进一步挖掘c-di-GMP信号-效应蛋白互作对生物胺的调控机制，最终构建关键产胺菌K. oxytoca生物胺-氨基酸脱羧酶基因簇-c-di-GMP信号相互关联的生物胺分子调控网络。本研究针对水产食品生物胺形成与积累过程中的共性关键科学问题，以易腐鲭鱼为研究对象，旨在明确鲭鱼生物胺有效控制的关键靶点，提升鲭鱼的加工率和加工品质，有助于丰富水产食品贮藏保鲜理论，保障消费者的食用安全。

The effectively control of biogenic amines is the key to the sustainable development of perishable aquatic food industry such as mackerel. Many studies have shown that microorganisms play an important role in the formation and accumulation of biogenic amines. Further investigation found that K. oxytoca was a key amine-producing bacterium of mackerel. The amine-producing ability of K. oxytoca was also positively correlated with the signal intensity of cyclic diguanylate (c-di-GMP). In view of this, the formation and accumulation mechanism of biogenic amines is to be revealed based on the c-di-GMP signal metabolic pathway through the composition analysis of amino acid decarboxylase gene cluster and its role in K. oxytoca biogenic amines formation. The regulation mechanism of c-di-GMP signal-effector protein interaction on biogenic amines formation is further to be explored. Finally, the biogenic amine molecular regulatory network of biogenic amine-amino acid decarboxylase gene cluster-c-di-GMP signal correlation is to be constructed in K. oxytoca. The purpose of this study is to solve the common key scientific problems in the formation and accumulation of biogenic amines in aquatic food. The perishable mackerel is treated as the research object in the present study. The key targets for the effective control of biogenic amines in mackerel should be identified and the processing rate and quality should be improved. The whole project will help to enrich the theory of aquatic food storage and preservation and ensure the safety of consumers.