CpG寡聚脱氧核苷酸通过调控PI3K/AKT信号通路介导的糖代谢诱导对虾训练免疫的作用机制

病害频发严重制约对虾养殖产业的健康发展，增强对虾自身免疫力可从根本上防治病害。免疫增强剂可诱导对虾产生训练免疫来增强其自身免疫力。CpG寡聚脱氧核苷酸(CpG oligodeoxynucleotides，CpG ODNs)是一种应用前景广阔的新型免疫增强剂。我们已发现CpG ODNs对凡纳滨对虾的免疫保护作用可能与PI3K/AKT信号通路介导的糖代谢改变有关。基于此，本项目拟继续在凡纳滨对虾中进行① CpG ODNs对训练免疫的诱导、② 糖代谢对CpG ODNs诱导训练免疫的响应、③ CpG ODNs诱导训练免疫中PI3K/AKT信号通路的功能、④CpG ODNs对PI3K/AKT信号通路的调控等研究，以期揭示CpG ODNs通过调控PI3K/AKT信号通路介导的糖代谢来诱导对虾产生训练免疫的作用机制。研究结果有助于建立对虾类动物病害的免疫防治技术，也可为无脊椎动物免疫学研究提供理论依据。

The frequent occurrence of diseases has restricted the healthy development of the shrimp culture industry. Enhancing the self immunity of shrimp can prevent and control disease fundamentally. Immunopotentiator can induce the trained immunity of shrimp to enhance their own immunity. CpG oligodeoxynucleotides (CpG ODNs) is a new immunopotentiator with wide application prospect. In our previous study, it was found that the immunoprotective effect of CpG ODNs on Litopenaeus vannamei may be related to the changes of glucose metabolism mediated by PI3K/AKT signaling pathway. On this basis, this study will try to reveal the mechanisms of the induction of trained immunity by CpG ODNs through regulating the PI3K/AKT signaling pathway mediated glucose metabolism in L. vannamei as the following aspects: ① Induction of trained immunity by CpG ODNs, ② The response of glucose metabolism to the induction of trained immunity by CpG ODNs, ③ The function of PI3K/AKT signaling pathway in trained immunity induced by CpG ODNs, ④ The regulation of PI3K/AKT signaling pathway by CpG ODNs. The results are helpful for the establishment of immune control technology for shrimp diseases, and also provide theoretical reference for the immunological research of invertebrates.