木材发育是多基因参与、呈网络调控、极其复杂且高度有序的生物学过程。研究其分子机理，阐明未知基因功能对于利用基因工程培育林木新品种具有重要意义。然而，由于树木遗传杂合度高、生长周期长，遗传学分析困难，造成不能简单快捷利用树木自身突变体分析其基因功能。因此急需寻求快速简便方法鉴定木材发育相关基因功能，阐明木材发育分子机理。研究发现拟南芥经次生生长诱导后可形成大量次生维管系统，与木材发育相似。因此，本研究利用拟南芥次生生长诱导生长体系，以大量木材发育相关候选基因为基础，由国际拟南芥资源库获得同源基因拟南芥突变体，快速发现表型变化及生理变化突变体，由大量候选基因中快速锁定目标基因，利用基因工程技术研究目标基因功能及调控关系，阐明目标基因在木材发育中的分子机理。

Wood formation is multi-gene involved, net-regulated, very complicated and ordered bilogical process. It is very important in tree's breeding to use DNA recombination technology for researching molecular mechanism and identifing genes' functions of wood formation. However, tree genes' function cannot be identified quickly by trees mutants because of tree's high heterozygosity, long life cycle and difficlult genetic analysis. It is necessory to find a new and simple method in identifing genes' function and explaining genes' molecular mechannism. Some research results showed that secondary induction to Arabidopsis thaliana can develope much secondary vascular tissues such as wood strcture. Therefor, this research plan would use Arabidopsis thaliana secondary induction system to culture these arabidopsis mutants which are from the international Arabidopsis Mutants Stock Centre and then identify the phenotypic or physiological changed mutants on the basis that much more wood formation involved genes are known. At last, this research plan can find interest genes quickly and identify the genes' function and regulational mechanism, then explaining genes' molecular mechannism during the wood formation.