基于SPME活体采样技术研究药物对生物内源性活性物质的影响机制

药物污染是近年来备受关注的环境问题，开展药物的环境行为和生物效应研究具有重要科学意义。已有研究表明药物污染可影响生物体的生长发育，但从分子水平研究药物对生物内源性活性物质的影响机制仍亟需深入开展。活体SPME是一种新型非破坏性采样技术，能实时跟踪内/外源性物质在生物体内的浓度水平，从而更准确、真实地反映不同物质间的潜在影响。本项目拟利用活体SPME技术研究药物对生物内源性活性物质的影响机制：合成新型功能材料，制备高选择性、高萃取容量的SPME探针；建立对目标分析物的高灵敏SPME活体采样分析方法，系统研究药物的环境过程和生物效应，探明药物对内源性活性物质的调控规律；同步测定酶活变化、基因表达、DNA损伤等指标，阐明药物对内源性活性物质的影响机制。本研究可为生物体内/外源性物质的跟踪监测提供新方法、新思路，有助于更深入认识药物的生物效应，对评价药物滥用等带来的生态风险和健康风险具有重要意义。

Pharmaceutical contamination has emerged as a new environmental problem that attracting wide attentions recently. Thus, investigating the environmental behaviors and biological effects of pharmaceuticals is of significantly importance. To date, several researches have revealed that pharmaceutical contamination could influence the growth of biotas. However, studying the impact mechanism of pharmaceuticals on important substances, especially the biological endogenous active substances (BEASs) in living bodies is still scarce. In vivo SPME, as a novel nondestructive sampling technique, is capable of simultaneously real-time monitoring both exogenous and endogenous substances in living organisms, sequentially revealing the potential effects of different substances more accurately. The project is to investigating the impact mechanism of pharmaceuticals on BEASs by using in vivo SPME technique. Firstly, a series of new functional materials will be designed and engineered to fabricate novel SPME fibers with high selectivity and extraction capability. Subsequently, establishing high-sensitivity in vivo SPME sampling and analytical methods for tracing pharmaceuticals and BEASs that enables to study the environmental behaviors and biological effects of pharmaceuticals, and then elucidating the regulation rules of pharmaceuticals to BEASs. Finally, the parameters such as enzyme activity changes, gene expression levels and DNA damage extents in living organisms, will be measured at the same time, which will uncover the influence mechanisms of pharmaceuticals on BEASs. The project can not only provide new techniques and strategies for monitoring of exogenous pharmaceuticals and BEASs in biotas, but also be helpful to in-depth understand the biological effects of pharmaceuticals, which is significantly important to evaluate the ecosystem/human health risks of pharmaceutical abuse.