甲维盐诱导舞毒蛾中肠细胞程序性死亡作用机制研究

舞毒蛾是一种世界性害虫，中肠是其主要消化器官，也是杀虫剂毒性作用的重要靶标。甲维盐对鳞翅目具有高效、低毒杀虫活性的杀虫剂。前人研究发现该杀虫剂能诱导害虫ROS大量生成，过量ROS引起细胞损伤，诱导细胞凋亡和程序性坏死，从而提高了杀虫效果。项目组在前期研究基础上：围绕甲维盐对舞毒蛾毒性作用机理这一中心科学问题，另辟蹊径，通过分子生物学等相关技术方法，将开展：（1）甲维盐对舞毒蛾的毒力损伤研究；（2）甲维盐暴露对NADPH/ROS通路影响研究；（3）NADPH/ROS通路在甲维盐诱导舞毒蛾中肠细胞凋亡和程序性坏死分子机制研究。以期证实舞毒蛾中肠细胞凋亡和程序性坏死是甲维盐杀虫的毒理学基础。阐明NADPH/ROS通路在甲维盐诱导舞毒蛾中肠细胞凋和程序性坏死的分子机制。为开发高效低毒农药提供理论依据。

Gypsy moth is a worldwide pest. Its midgut is the main disgestive organ, which is also an important target of insecticide toxicity. Emamectin benzoateis is a kind of insecticide with high efficiency, low toxicity and high insecicidal activity to Lepidoptera. Previous researchers have found that insecticides can increase the production of ROS. Excessive ROS can cause cell damage and induce apoptosis and programmed necrosis. Abnormal and excessive apoptosis can cause a cariety of tissue and organ damage. Based on the previous studies, our project team will focus on the scientific issue of NADPH/ROS pathway' s role in apoptosis and programmed necrosis in the midgut of gypsy moth exposed to emamectin benzoate, and carry out: (1) Determination of virulence to gypsy moth by emamectin benzoate; (2) studies on the effect of emamectin benzoate on NADPH/ROS pathway in gypsy moth; (3) studies on the molecular mechanism of NADPH/ROS pathway in midgut cell apoptosis and programmed necrosis induced by emamectin benzoate in gypsy moth. The expectation is to confirm that midgut cell apoptosis and programmed necrosis is the toxiclolgical basis of emamectin benzoate insecticide, and elucidate the molecular mechanism of NADPH/ROS pathway in midgut cell apoptosis and programmed necrosis induced by emamectin benzoate in gypsy moth. It will provide a new theoretical basis for the development of insecticide with high efficiency and low toxicity.