RNAi介导的舞毒蛾JHEH基因功能及代谢调控机制研究

保幼激素(juvenile hormone,JH)是一种倍半萜类衍生物。保幼激素环氧化物水解酶（JHEH）作为保幼激素降解的的酶类，在昆虫的整个生长发育过程中起重要作用。前期我们克隆获得舞毒蛾JHEH基因，其在舞毒蛾不同虫态均有表达差异，通过喂食JHEH的dsRNA证实能够显著降低JHEH基因的表达量。围绕JHEH如何参与保幼激素的代谢与信号传导这一科学问题，通过分子生物学相关技术，将开展如下研究内容:（1）舞毒蛾JHEH基因特异性表达（2）JHEH对舞毒蛾生长发育影响（3）在保幼激素代谢中JHEH、JHE和JHDK三者间的协同作用（4）JH信号转导通路分析（5）保幼激素及蜕皮激素含量测定。通过以上研究阐明舞毒蛾JHEH基因重要生理功能，说明JHEH基因在舞毒蛾幼虫体内保幼激素代谢、信号转导及成虫翅型分化中参与的激素代谢调节机制，为新杀虫剂的创制研究提供了一个潜在的有价值的新靶标。

The gypsy moth, Lymantria dispar, is a key lepidopteran species that has been estimated to colonize at least 300 different forest species worldwide. Juvenile hormone (JH) as a group of sesquiterpenoids biosynthesized by the corpora allata is an important insect hormone in the haemolymph that regulates insect larval development, reproduction, and metamorphosis. Juvenile hormone epoxide hydrolase (JHEH) is an important enzyme in the degradation pathways of juvenile hormone (JH) in insects. JHEH titers regulate the entire process of insect development. Now, we had cloned a JHEH gene of gypsy moth. The transcriptional profiles of JHEH were detected by qRT-PCR and found that it was expressed throughout all stages. JHEH gene was successfully knocked down by dsRNA in the instar larvae. In order to known the function of JHEH in the JH degradation and JH signaling pathway. In this project, we will conduct the following studies to explore the functions of JHEH gene in gypsy moth including (1) the specific expression of JHEH in gypsy moth; (2) the effects of silencing JHEH gene on the development of gypsy moth; (3) The synergy of JHEH, JHE and JHDK in JH degradation; (4) characterization of JH signal transduction of JHEH in gypsy moth; (5) the contents of juvenile hormone and molting hormone are determinated by HPLC. These results will demonstrate physiological functions of JHEH gene in JH degradation, JH signal transduction and wing form of gypsy moth. In addition, it will provide a new way for formulate new pesticide based on finding key genes participated in important physiological function.