生物质炭负载噬菌体对土壤中抗生素耐药菌(Pseudomonas aeruginosa)迁移阻控及靶向裂解的协同机制

土壤抗生素耐药菌(Antibiotic Resistant Bacteria，ARB)残留风险已严重威胁人类健康和环境安全,开展针对性修复研究十分必要。国内外学者发现高效阻控材料生物质炭可显著抑制土壤中ARB迁移扩散，但生物质炭未能消除土壤ARB持续增殖风险。噬菌体可专属裂解环境中宿主细菌，因此生物质炭负载噬菌体为长效靶向修复ARB污染土壤提供了新的途径。本项目拟选用铜绿假单胞菌(Pseudomonas aeruginosa PAO1)作为模式耐药菌，将生物质炭高效阻控特性与噬菌体专属裂解属性有机结合,制备生物质炭负载噬菌体材料，在探明负载作用机理的基础上，运用荧光蛋白示踪、宏噬菌体组学、共现网络模型等技术，揭示负载材料对土壤抗生素抗性PAO1迁移阻控及靶向裂解的协同作用原理，为生物质炭负载噬菌体修复ARB污染土壤提供科学指导。

As residual antibiotic resistance bacteria (ARB) in soil have posed serious threat against the public health and environment security, it is urgent to carry out targeted remediation research. Previous studies found that environmentally friendly biochar could significantly inhibit the transmission of ARB, and bacteriophages (phages) were capable of lysing host bacteria in the environment. Therefore, biochar loaded phage complex provides a novel long-lasting pathway of precisely remediating ARB-contaminated soil. In this proposal, a novel biochar-loaded phage material will be developed through physico-chemical adsorption and bioburden to accelerate the dissipation of antibiotic-resistant Pseudomonas aeruginosa PAO1 in soil. In addition, the underlying mechanism of synergistic effect of biochar and phage will be further investigated via fluorescent protein tracing, phage and bacteria metagenomics, and co-occurrence network model analyses. The results obtained here will shed light on the development of novel sustainable technology for remediating ARB-contaminated soils.