兽药的滥用导致了在我国许多畜禽养殖场周边出现了大面积抗生素-抗性基因(antibiotic resistant genes, ARGs)复合污染农田土壤，给人体健康和生态环境安全带了较严重隐患，开展针对性修复研究十分必要。国内外学者对好氧条件下土壤抗生素降解已有较为系统研究，但在厌氧环境中，由于受到氧气、还原反应体系、电子受体和微生物活性等因素限制，抗生素-ARGs消减研究进展缓慢。本项目拟在纯厌氧环境中，硝酸盐还原体系下，从调节电子受体浓度(硝态氮添加量)和促进抗生素微生物可利用性(生物表面活性剂槐糖脂添加量)的角度，探究反硝化强度与槐糖脂对土壤抗生素-ARGs同步厌氧消减的交互作用，探明决定消减进程的关键因素，阐释消减过程中抗生素厌氧降解菌群、抗性细菌菌群及反硝化细菌菌群在群落生态功能上的响应机制，为反硝化作用与槐糖脂协同强化土壤抗生素-ARGs厌氧消减修复提供理论指导和科学依据。

Unregulated application of veterinary antibiotics in animal husbandry industry has resulted in fairly amount of antibiotic/antibiotic resistant genes (ARGs) mixed contaminated farmland soils in China, which has also caused potential threaten against human health and ecological safety. Therefore, it has been becoming a necessity to carry out targeted remediation. So far, antibiotic degradation in aerobic soils has been systematically carried out by previous researchers. Degradation research in anaerobic, though equally important, which were much less studied due to more complex impacting factors, such as oxygen deficiency, and ariance in reduction systems, electron acceptors, antibiotic classification and bioaccessibility. In this proposal, an anaerobic system (nitrate reduction system) will be designed to study the role of factors including nitrate reducing strength (nitrate concentation) and antibiotic bioaccessibility (sophorolipid addition) in prompting the anaerobic dissipation of soil antibiotics and ARGs. Moreoever, the Surface Response Model, a Three Compartment Desorption Model, 13C stable isotope method, and high throughput sequencing technology will be employed to explore the main factors that limit the dissipation of mixed pollutants; illucinate the microbial ecological response mechanism of antibiotic degradating bacteria, antibiotic resistant bacteria, and denitrifiers. The study will provide theoretical direction and scienfitic bases for better understanding the sophorolipid-enhanced soil antibiotic/ARGs dissipation in denitrifying condition.