双层纳米银/生长因子缓释型多功能敷料的构建及其用于皮肤损伤修复的研究

传统的伤口敷料和皮肤移植是针对创面处理最常用的治疗方法，但它们只起到暂时的屏障作用，缺乏抗菌以及主动诱导组织再生修复的能力。因此，如何封闭创面、防治创面感染以及加快创面愈合是目前新型功能性敷料研制的重点。本研究基于申请人对组织工程生物材料的前期基础研究优势，采用药物缓释体系的多级构建策略，探讨双组份药物（纳米银/bFGF）缓释复合功能性敷料的构建思路和方法，使构建的双层敷料兼具有抗感染和加速组织再生的功效。研究利用微球系统、真空冷冻干燥法和乳液静电纺丝技术制备双层载双组份纳米银/bFGF的海藻酸钠/壳聚糖-PLGA/羊毛角蛋白复合敷料，并对其理化性能、药物释放、细菌穿透性能、抑菌性能、细胞毒性等进行表征，评价其安全性（皮肤急性毒性、皮肤刺激性以及皮肤过敏性实验），最后观察其对皮肤创面的修复能力，以期为具有药物缓释功能的复合功能性敷料的开发以及皮肤创伤治疗提供实验基础和理论依据。

Traditional wound dressing and skin transplantation are the most commonly used methods for wound treatment, but they only play a function of mechanical barrier and isolation, lacking of antibacterial and active induction of tissue regeneration ability. Therefore, how to seal the wound, prevent the wound infection and accelerate the wound healing are the key points in the development of the new functional dressing. In this study, based on the strategy of drug delivery system, the ideas and methods of building a two-component drug (nano-silver/bFGF) released composite functional dressing will be explored, which haves the function of anti-infection and acceleration of tissue regeneration. A bilayered nano-silver/bFGF sodium alginate/chitosan-PLGA/ wool keratin composite dressing will be prepared by microsphere system, vacuum freeze-drying method and emulsion electrospinning technology. Their physical and chemical properties, drug release behavior, antibacterial ability, cytotoxicity, safety in vivo (acute skin toxicity, skin irritation, and skin irritability tests) will be characterized. Then, the bilayered composite dressing will be used to evaluate the ability to repair skin damage. The study will serve as a foundation for further study of drug delivery functional dressing and the therapy of skin wound.