具有拓扑形貌的双层引导组织再生膜的构建及其细胞响应行为研究

引导组织再生(GTR)及牙周组织工程技术为牙周病的治疗带来了全新的理念并取得了确实的临床效果。GTR膜/支架材料是这两项技术的关键。通过改变材料表面的拓扑形貌对其进行改性，调控细胞行为，激活细胞功能和基因的表达是目前生物材料设计的重要思路之一。本研究基于申请人对引导组织再生材料的前期基础研究优势，依据细胞-材料相互作用的思路，制备了一系列具有不同微米表面拓扑形貌（微柱、微坑和沟槽）的双层PLGA/羊毛角蛋白引导组织再生膜，检测其表面形貌、力学性能、对骨髓间充质干细胞和牙周膜干细胞黏附、分化等行为的影响、体内生物相容性等，最后用于GTR术，观察复合膜对牙周组织的再生作用，以期为具有拓扑形貌的理想引导组织再生膜的开发、研究其对干细胞行为的影响以及牙周病的再生治疗提供实验基础和理论依据。

Guided tissue regeneration (GTR) and periodontal tissue engineering technology have brought a new concept for the treatment of periodontal disease and achieved a real clinical effect. GTR membrane/ scaffold material are the key factors of these two technologies. At present, it is one of the important ways to improve the design of the biomaterials by changing their surface topography, regulating cell behavior, and activating cell function and gene expression. Based on the idea of cell-material interaction, in this study, a series of bilayered PLGA/wool keratin composite membranes with different micrometer surface topography (post, pit and groove) will be prepared. The surface morphology, mechanical properties, the effect on the behavior of BMSCs and PDLSCs, and the biocompatibility in vivo will be investigated. Then, the composite membranes will be used to evaluate the effects in guided tissue regeneration in rabbits. The study will serve as a foundation for further study of GTR membranes with surface topography, the effect on stem cell behavior and the therapy of periodontal disease.