艾比湖湿地盐生植物根际粘细菌资源、多样性及其生物地理区系特征

本项目立足于艾比湖湿地在科、属、种水平上分别占中国盐生植物42.5%、30.5%和25.4%的植被特色，以建群盐生植物根际土壤微环境为切入点。采用传统粘细菌筛选和纯化方法分离粘细菌,通过对其形态学特征、生理生化特性、DNA G+C mol%、16S rRNA和HSP60基因序列等方面研究，分类鉴定粘细菌菌株。基于可培养粘细菌16S rRNA和HSP60基因序列构建的系统发育树，认识艾比湖湿地可培养粘细菌资源及多样性。根据粘细菌亚目的16S rDNA序列和HSP60持家基因序列设计特异引物，对盐生植物根际土壤总DNA进行高通量测序，富集未培养粘细菌。构建盐生植物根际未培养粘细菌系统发育树，分析未培养粘细菌群落结构、种群组成、优势菌群特点。结合各盐生植物根际微环境特性，探讨可培养和未培养粘细菌资源的生物地理区系特征，科学认识艾比湖湿地极端环境盐生植物根际粘细菌资源的生态多样性特点。

This project is based on the vegetation characteristics of Ebinur Lake wetland, which belong to 42.5%, 30.5% and 25.4% of halophyte in the family, genera and species level, respectively, and takes the rhizosphere soil microenvironment of colonization halophyte as the breakthrough point. The traditional screening and purification methods of myxobacteria were used to isolate myxobacteria, and the myxobacteria strains were identified by the morphological features, physiological and biochemical characteristics, genomic DNA G+C mol % , 16 S rDNA sequence and HSP60 gene sequence. By using the phylogenetic tree plotted by the 16S rDNA sequence and HSP60 gene sequence of the culturable myxobacteria, we could understand the distribution characteristics of culturable myxobacteria in Ebinur Lake wetland. Specific primers were designed according to the sequence structure of 16S rRNA and HSP60 gene, and high-throughput sequencing of total DNA from rhizosphere soil of halophyte was used to enrich uncultured myxobacteria in halophyte rhizosphere. The phylogenetic tree of the uncultivated myxobacteria in the rhizosphere of halophytes was constructed, and the community structure, population composition and dominant bacteria group characteristics of the uncultured myxobacteria were analyzed. Combined with the characteristics of rhizosphere microenvironment of different halophytes, we explored the biogeographic flora characteristics of culturable and non cultured myxobacteria, and scientifically understood the characteristics of ecological diversity of myxobacteria resources from the halophytes rhizosphere soil in the extreme environment of the Ebinur Lake wetland.