本研究立足于国内最具代表性的温带干旱地荒漠盐化生态系统——新疆艾比湖湿地微生物资源优势，以湿地主要代表性植物物种根际与非根际土壤、底泥为介质，以影响湿地生态功能的关键循环——氮循环中的固氮作用、硝化作用、反硝化作用微生物功能群功能基因为切入点，以根际微环境自然态的氨态氮、硝酸态氮、亚硝酸态氮、可溶性总氮、可溶性有机氮、固定态铵为目标化合物，采用将克隆文库构建、实时定量PCR、环境理化学因子数据、不同形态氮化合物含量相结合的分析方法，研究参与根际氮循环的固氮菌群落、氨氧化细菌群落、氨氧化古菌群落和反硝化细菌群落的种群组成、分布、数量丰度动态变化、结构差异对不同植物物种根际微环境的响应，探讨氮循环关键过程微生物功能群群落结构的生态位分化特征及其在不同植被环境中可能的功能活性差异。这对评价与预测由氮循环微生物驱动的艾比湖湿地不同植被种植区的生态功能恢复效益、制定湿地管理保护决策有重要价值。

This study based on the microbial resource advantage of Xinjiang Ebinur Lake Wetland which is the desert saline ecosystem of the most typical temperate arid in our country. Choosing the rhizosphere and non-rhizosphere soil of the wetland main plant species as the research medium. With nitrogen fixation, nitrification and denitrification functional gene of microbial functional groups of nitrogen cycle of the key cycle of wetland ecological functions as the breakthrough point. With NH4+-N, NO3--N, NO2--N, soluble nitrogen, soluble organic nitrogen, fixed ammonium of rhizosphere micro-environment as the target compounds. Adopting the analysis method of clone library building, real time quantitative PCR combined with physical and chemical factor data and nitrogen compound content of different forms. We will study the population composition, distribution, abundance dynamics and structural differences of nitrogen-fixing bacteria and ammonia-oxidizing bacteria and ammonia oxidizing archaea and denitrifying bacterial community involved in nitrogen cycle of rhizosphere in responses to the rhizosphere and non-rhizosphere environment of different plant species. Our research is to explore the ecological niche differentiation characteristics of microbial functional groups community of nitrogen cycle key process and their possible functional activity differences in different vegetation environments. This research has the important value to evaluate and forecast the ecological functions recovery efficiency of different vegetation growing region of Ebinur Lake wetland, and to develop wetland management and protection decisions.