寒证阳痿病证模型与药、灸干预的阴茎组织特异性蛋白表达与调控研究

前期工作中，我们按照病因造模理念，创建了寒证证候与阳痿病证模型，并从阴茎组织水平明确91种病证相关差异表达蛋白质。本研究拟复制该证候与病证大鼠，通过自然反证和艾灸反证从证候、病证两个层面对模型进行系统评价。随后以伊木萨克提取物和艾灸（关元穴）干预，通过下丘脑、垂体、肾上腺、甲状腺、睾丸、阴茎等组织、器官的病理学观测和“NEI”相关激素、内皮与平滑肌相关指标检测，明确模型“NEI”和阴茎组织病理生理学改变特征与药、灸调控机制。随后构建候选蛋白标志物，对干预前后的阴茎内皮、平滑肌等效应组织进行蛋白质水平定量验证，明确病证蛋白标志物与药、灸作用靶点，并结合生物信息学分析，进一步构建、检测特异性网络中蛋白标志物上下游关键指标，探讨阳痿病证模型与药、灸作用机理的关系，从NEI网络-整体和阴茎组织-局部两个层面诠释阳痿病证发生的生物学本质及药、灸的调控机理, 揭示“病证结合”、“方证相应”科学内涵。

In our previous research, we established impotence model with Han syndrome according to the concept of etiology modeling and then identified 91 differentially expressed proteins related to impotence disease syndrome. By copying the impotence model with Han syndrome，this study intends to carry out natural counter-evidence and moxibustion (Guan yuan acupoint) counter-evidence from the point of Han syndrome and impotence disease syndrome. Then, we aim to clarify the "NEI" network and penile tissue pathophysiology changes and characteristics, medicine and moxibustion regulation mechanism by studying the regulation mechanism through the pathological observation of tissues and organs such as hypothalamus, pituitary gland, adrenal gland, thyroid gland, testis, penis and related indexes of "NEI" network related hormones, endothelium and smooth muscle detection after treating with Yimusake extracts and moxibustion. Furthermore we aim to construct and detect the upstream and downstream key indicators of protein markers in the specific network combining with bioinformatics analysis to explore the relationship between the model of impotence syndrome and the mechanism of medicine and moxibustion, and to interpret the biological essence of impotence syndrome and regulation mechanism of medicine and moxibustion from the prospect of NEI network-as whole, and penile tissue-as local, and to reveal the scientific connotation of the "combination of disease and syndrome" and "correspondence between prescription and syndrome".