异常黏液质型阳痿病证及其方药干预与体内蛋白表达调控的关系研究

阳痿是维医优势病种，其主要证型是异常黏液质。前期工作中，我们通过异常黏液质型阳痿病证及其方药伊木萨克片干预的血清蛋白质组学研究，发现23种共性候选蛋白质标志物。本研究拟以上述蛋白质为指标，对阳痿患者及动物模型血清进行定量分析，明确临床和动物模型的共同指标；利用上述动物模型及其方药干预，收集下丘脑、垂体、睾丸、阴茎等阳痿病证的潜在靶器官组织，通过候选蛋白质表达和蛋白质磷酸化分析，明确血清水平的整体变化与特定组织内基因表达和调控的关系；收集阳痿病证动物模型及其方药干预后血清，对阴茎海绵体内皮细胞进行干预，通过候选蛋白质水平及内皮功能指标分析，探讨阳痿病证及方药干预对内皮细胞功能的影响。通过本研究，建立阳痿病证的蛋白质标志物，方药作用的潜在靶点，从整体观探讨阳痿病证发生与多系统和组织功能异常变化的关系及方药的多靶点作用机制，为诠释“病证结合”、“方证相应”等维医理论和临床实践的科学性提供依据。

Impotence is a predominant disease in Uygur Medicine, and its main type is abnormal phlegmatic syndrome. In our previous study, we investigated the serum proteomics of abnormal phlegmatic syndrome of impotence disease with and without drug intervention with Yimusake and found 23 protein as common candidate biomarkers. In this study, we will quantify the expression of these proteins in serum of impotence patients and animals to identify common index shared by clinical patients and animal models. Using the above animal models with and without drug intervention, we will collect potential target organs of abnormal phlegmatic syndrome, such as hypothalamus, pituitary, testis, penis. According to the candidate protein expression level and protein phosphorylation level in these organs, we will define the relationship between the changes of serum protein in the whole body and gene expression and regulation in the target organs. Furthermore, we will treat corpora cavernosa endothelial cells with the serum of animals with abnormal phlegmatic syndrome of impotence disease with and without drug intervention. By analyzing the candidate protein levels and endothelial functions, we are able to explore the effect of syndrome and drug intervention on target cell function of impotence syndrome. In the present study, we are going to establish protein biomarkers of impotence disease syndrome, to find the potential target for drug treatment, and to explore the relationship between the occurrence of impotence disease and the abnormal changes in functions of multiple systems and organs as a whole body. The present study will provide the scientific basis for the Uygur Medicine theory and clinical practice about "combination of disease and syndrome "and " prescriptions corresponding to syndromes ".