植物内生真菌(endophytic fungi)作为重要的天然活性物质资源宝库，能够产生多种类型的生物活性物质，在植物的生长、代谢和抗病性等方面具有重要的生理和生态功能。桉树（Eucalyptus spp.）是我国华南地区重要的经济林和生态林树种，而桉树青枯病的大面积发生严重制约着桉树产业的发展。本项目拟从桉树中分离筛选具有抗桉树青枯病菌（Ralstonia solanacearum）活性的内生真菌，并分离鉴定内生真菌产生的抗菌活性成分，研究其抗菌作用机制，从而为抗青枯病菌药物的研制提供先导结构；同时探讨桉树、内生真菌和青枯病菌三者之间的相互作用关系，为内生真菌在桉树青枯病生物防治领域的开发和利用奠定基础。

As important natural resources, endophytic fungi, which produce various types of bioactive substances, play important physiological and ecological functions in plant growth, metabolism and disease resistance. Eucalyptus spp. are important economic and ecological trees species in southern China. However, the development of eucalyptus industry are seriously restricted by the occurrence of the bacterial wilt in large area. The proposed project intends to isolate and screen endophytic fungi which show antagonist effect on Ralstonia solanacearum from Eucalyptus spp., separate and identify the antibacterial active ingredients from active endophytic fungus, and study the antibacterial mechanism which would lead to provide the forerunner in development of anti-bacterial wilt drugs. The interactions among Eucalyptus spp., endophytic fungi and Ralstonia solanacearum will also be investigated in this project, which will lay a foundation for development and utilization of endophytic fungi in the field of biological control.