荷斯坦母牛安静发情体温变化规律及其激素调控机制研究

安静发情是奶牛养殖业一直悬而未决的科学问题，给奶牛养殖业造成了巨大经济损失。而奶牛发情尤其是安静发情伴随的体温升高现象，为安静发情鉴定技术研发带来希望。本团队前期研究发现，安静发情和正常发情牛体温均大幅、持久、平稳增加，结合前人报道重要线索，体温变化规律研究极有可能揭示安静发情变化规律。为此，本研究拟通过检测分析发情荷斯坦母牛体温、活动量、卵泡状态和相关生殖激素变化，针对发情、排卵的不连续性状，分别建立基于分类变量的多分类逻辑回归和基于连续变量的梯度下降预测模型，综合预测分析体温、活动量的发情排卵规律，比较和优化预测效果；并深入揭示安静发情、排卵母牛体温、活动量等因素变化的激素调控机制，以促进母牛安静发情鉴定技术取得根本突破。

Quiet estrus is an unsolved scientific problem in the dairy cattle industry, which has caused huge economic losses. The increase of body temperature during estrus, especially the quiet estrus, brings hope to develop identification technology for cow’s quiet estrus. Our previous research found that the body temperature of cow in quiet estrus or normal estrus increased significantly, persistently and steadily. Combined with other important clues in this field, study on the fluctuation of body temperature is very likely to reveal the pattern of quiet estrus. Therefore, this project focuses on the alteration of Holstein cow’s body temperature, activity, follicle status and the related reproductive hormones. According to the discontinuous characteristics of estrus and ovulation, the prediction models of multi-classification logistic regression based on classification variables and gradient descent based on continuous variables were established, and the rules of estrus ovulation based on body temperature and activity level were comprehensively predicted and analyzed to obtain an optimized prediction effect. Furthermore, the hormone regulation mechanism of the regularity of body temperature and activity level during quiet estrus and ovulating in cows will be explored to promote the fundamental breakthrough in the identification of quiet estrus in cows.