多重界面组装仿鳞构筑鱼胶原基“硬核”多功能口腔GBR膜及界面强化机制

胶原基引导骨再生（GBR）膜是口腔种植修复手术的关键核心材料，但目前存在哺乳动物胶原原料风险与限制、膜功能单一、膜易塌陷和破裂等瓶颈问题。本项目围绕制约胶原基GBR膜临床应用的基础问题“膜的高稳定性与可控降解性的协调”，提出以机械性能优异的鱼鳞Bouligand结构为仿生模板，通过取向电纺、仿生矿化、填充黏合等仿生策略，采用聚合物交联、成核诱导等界面强化手段，基于多重界面组装仿鳞构筑高性能、多功能的GBR膜。拟阐明开环易位聚合大分子原位交联、类沸石咪唑酯骨架原位矿化、胶原自组装等分别对胶原分子界面、矿化胶原有机/无机界面、胶原纤维界面等多界面的强化机制；揭示多重界面耦合与GBR膜结构性能的关系，实现对膜稳定性与降解性的同步调控，设计构建集屏障支撑、抗菌活性、骨诱导性等功能于一体的新型鱼胶原基“硬核”多功能GBR膜。本项目可为鱼加工废弃物向口腔医用耗材的高值转化利用提供重要的理论与实践基础。

Collagen based guided bone regeneration (GBR) membrane is the key core material for oral implant repair, but there are some bottleneck problems such as the risk and limitation of collagen raw materials, single membrane function, membrane collapse and rupture. In this project, focusing on the basic problem of restricting the clinical application of collagen based GBR membrane "the coordination of high stability and controllable degradation of the membrane", it is proposed to take the Bouligand structure of fish scales which has excellent mechanical properties as the biomimetic template. By using the biomimetic strategies such as oriented electrospinning, biomimetic mineralization, filling and adhesion, and other interfacial strengthening means such as polymer crosslinking and nucleation induction, the GBR membrane having high performance and multifunction can be constructed based on multiple interfacial assembly. It is proposed to clarify the strengthening mechanism of in-situ cross-linking of polymer based on Ring-Opening Metathesis Polymerization, in-situ mineralization of Zeolitic-Imidazolate-Framework-8 and collagen self-assembly on the multi-interfaces of collagen molecular interface, organic/inorganic interface of mineralized collagen, and collagen fiber interface. The project is to reveal the relationship between multi-interface coupling and the structure and performance of GBR membrane, to realize the synchronous regulation of membrane stability and degradation, and to design and construct a new type of fish collagen based “hard core” GBR membrane which has the functions of barrier support, antibacterial activity and bone induction. This project will provide an important theoretical and practical basis for the high-value transformation and utilization of fish processing waste to oral medical consumables.