肌骨交互作用及其共调节因子在冬眠期达乌尔黄鼠运动功能维持中的作用与机制研究

冬眠动物不仅是天然的抗废用性肌萎缩动物模型，还是良好的抗骨质疏松动物模型，阐明其独特的适应机制是生理生态学领域亟待研究的重要课题，对医学与航天科学领域废用性/失重性运动功能减退的防治亦具有重要借鉴意义。鉴于非冬眠动物的废用性肌萎缩与骨质疏松相伴发生，以及我们近期关于冬眠达乌尔黄鼠骨骼肌与骨骼代谢出现协同性变化的新发现，我们提出假说，冬眠期动物肌骨功能的协同维持可能得益于肌骨之间的交互作用，且受益于肌骨共同上游信号的调控。因此，本项目拟以达乌尔黄鼠为研究对象，对冬眠不同时期不同类型骨骼肌分泌的肌源性因子、骨骼分泌的骨源性因子及肌骨共调节因子的水平与作用机制展开研究，结合分析废用状态下冬眠动物与非冬眠动物（大鼠）相关指标的异同，首次尝试从一个全新的角度系统研究肌骨交互作用及共调节因子在冬眠期达乌尔黄鼠运动功能维持中的作用与机制，同时有望为人类废用性运动功能减退的整体化防治提供新思路。

Hibernators are not only a natural anti-disuse muscle atrophy model, but also a good anti-disuse osteoporosis model. Clarifying their unique environmental adaptation mechanisms is an important subject to be explored in the fields of animal physiological ecology, which is also of great significance for the prevention and treatment of disuse/weightlessness-induced motor function deterioration in the fields of spaceflight and medicine. In view of the concomitant occurrence of the disuse-induced muscle atrophy and osteoporosis in non-hibernators, as well as our new discovery upon the collaborative change of muscle and bone metabolism in hibernating Daurian ground squirrels, we hypothesize that, the cooperative maintenance of muscle and bone is likely to be benefit from the muscle-bone crosstalk, or the upstream co-regulators of these two organs during hibernation. Therefore, this project will focus on the levels and regulation patterns of the myokines, osteokines, as well as muscle-bone co-regulators at different hibernation stages, and combined with the comparative analysis of the related indexes between hibernators and non-hibernators (rats) under disuse condition. For the first time, a comprehensive analyze will be carried out from a very novel perspective to systematically explore the underlying mechanisms of the muscle-bone crosstalk and co-regulators in the musculoskeletal maintenance during hibernation, which also hopefully provide novel ideas for integrative therapeutic regimens for disuse-induced motor function deterioration.