一体化多重数字式病毒核酸精准现场检测系统

病毒的现场检测是控制疫情蔓延的关键技术。目前病毒核酸现场检测系统普遍存在检测时间长、自动化程度低及结果不够准确等缺陷。为了解决病毒现场检测的瓶颈问题，本项目拟研制一套集成核酸提取及多重数字重组聚合酶扩增（RPA）的自动检测装置，实现对病毒的高效、精准检测。首先，设计样品前处理微流控系统，结合磁珠法提取核酸，以此实现5分钟内自动化核酸提取；其次，由于新型多重数字RPA芯片设有阳性及阴性质控，通过单色荧光15分钟内能够实现六重数字检测；最后，通过微流控系统独特的连接管路设计、专门的控制系统、温控系统及检测系统，使得仪器实现一体化及小型化。一体化多重数字式病毒核酸现场检测系统通过将病毒检测的所有过程集成到一个小型装置中，可在20分钟内实现“样品进-数字化-结果出”的自动化检测过程。系统所具备的现场快速高灵敏精准定量检测性能，在未来应对病毒爆发时将发挥重要作用。

Point of care testing（POCT）of the viruses is the key technology to control the spreading of the outbreak. The current nucleic acid level detection systems generally have disadvantages including time-consuming, low-leveled automation, inaccurate results, etc. In order to solve the current bottleneck problem of POCT on virus, this project intends to develop a set of automatic detection devices that integrates the nucleic acid extraction and multiplex digital recombinant polymerase amplification (RPA) to achieve efficient and accurate detection of viruses. First, a microfluidic system for sample pretreatment is designed, combined with magnetic beads method to extract nucleic acids, to achieve extracting nucleic acid automatically within 5 minutes. Second, since the novel multiplex digital RPA chip has positive and negative controls, 6-plex digital detection can be finished within 15 minutes by using monochromatic fluorescence. Finally, through the unique design of pipeline connection, special control system, temperature control system and detection system of the microfluidic system, the instrument can be integrated and miniaturized. By integrating all the processes of virus detection into a small device, the system can perform "sample-in-digital-answer-out" method within 20 minutes. Since the system is fast, sensitive, accurate and quantitative in POCT, it is believed that the system will play an important role in virus outbreaks in the future.