土壤盐渍化是严重影响植物生长发育和农作物产量的重要非生物胁迫因素。土壤中高浓度的Na+会严重扰乱植物的正常代谢。研究表明，植物抵御盐胁迫的最有效的策略之一是通过Na+/H+逆向转运蛋白来调节植物体内的离子均衡。因此，利用转基因技术将具有高度耐盐性的Na+/H+逆向转运蛋白基因（NHX1，NHA1）转入到作物中培育出耐盐植物新材料对于农业生产具有重大意义。本研究通过寻找盐碱滩涂地的耐盐碱植物（耐盐幅度高达2.5%～4.0%）碱蓬、盐角草、大米草、星星草，分离克隆耐盐基因，并利用DNA shuffling 定向进化方法改造得到高耐盐性的NHX1、NHA1新基因，利用强耐盐改组新型基因转化拟南芥，分析苗期盐胁迫响应转录本表达谱差异及转录调控网络的关键控制单元，分析其对其他代谢途径（ABA途径）、信号传导途径的微调控影响，系统全面地探讨新型强耐盐碱改组Na+/H+转运蛋白基因的分子调控机理。

Soil salinization is an important abiotic stress factors with serious impact on plant growth and crop yield. Soil, with high concentrations of Na + would seriously disrupt the normal metabolism of plants. Previous studies have shown that one of the most effective strategies of plants to withstand salt stress is to adjust the ion balance of the plants through the Na + / H + antiporter. Therefore, the use of transgenic technology will have a highly impact plant salt tolerance by Na + / H + antiporter gene (NHX1, NHA1) activity and their transformation to breed new lines for salt-tolerant plants in the crop is of great significance for agricultural production. This study will focus on looking for the salinity tolerant plants of the saline tidal flat soils of about 2.5% to 4.0% salt content (Suaeda, Salicornia, English cordgrass, Alkali grass), from which to isolate and clone salt tolerance gene, and use DNA shuffling and other directed evolution methods to get high salt tolerance NHX1, NHA1 gene. Eventually, these new created gene will be transferred to the model plant Arabidopsis. Furthermore we will also study the transcript expression profile differences to analyze the transcriptional regulatory network of the key control unit. At last,we will analyze the impact of micro-regulation of other metabolic pathways (ABA pathway), signal transduction pathways, holistic approach to the reorganization of the new strong salinity of Na + / H + transporter gene molecular regulation mechanism.