多发性骨髓瘤细胞(MM)在骨髓微环境中与骨髓基质细胞(BMSCs)"对话"，在MM发病中起重要作用。STAT3信号通路是MM细胞与BMSCs相互作用的中心环节之一。最近我们首次报道中药活性成分小豆蔻明在体外具有良好的抗MM作用。近期我们进一步发现，小豆蔻明可抑制MM细胞中STAT3的活化。因此，我们推测小豆蔻明有可能通过抑制STAT3信号通路，阻断MM细胞与BMSCs相互作用，发挥多重抗MM效应。为验证这一设想，本项目拟从不同层次(分子、细胞、共培养、动物)，采用多种手段（EMSA、Western blot、ELISA、FCM、Real time PCR、免疫荧光等），系统观察小豆蔻明在MM细胞与BMSCs相互作用过程中对细胞生物学行为的影响，以及对STAT3通路的调控作用。本研究有望从全新角度阐明小豆蔻明抗MM的分子机制和作用靶点，为小豆蔻明进一步研究开发提供坚实的理论基础。

The crosstalk between myeloma cells and bone morrow stromal cells (BMSCs) in bone marrow milieu plays an important role in the development of multiple myeloma (MM). STAT3 pathway is one of the central factors during the interaction of MM cells and BMSCs. Currently we reported for the first time that cardamonin, an active component isolated from some Chinese herbs, possesses anti-MM efficacy in vitro. We further discovered that cardamonin inhibited STAT3 activation of MM cells. Therefore, we assume that cardamonin might exert its multiple anti-MM effect through inhibition of STAT3 pathway, thus blocking the interaction between MM cells and BMSCs. In order to confirm this proposal, we plan to systemically observe the influence of cardamonin on behavior of cellular biology during the interaction of MM cells and BMSCs, and the regulating effect on STAT3 pathway. This study may reveal the mechanisms and target effect of cardamonin from a brand-new aspect, and provide solid theoretical basis for further research.