酸/碱性等离子体活化液中RONS生成途径及其抗癌效应的定量解析与优化

近年来，大气压等离子体技术在癌症治疗方面展现出广阔的应用前景。目前等离子体灭活癌细胞研究主要考虑液相活性氧氮（RONS）的作用，却忽视了溶液pH在抗癌中的贡献。pH严重控制着RONS生成途径，最终影响到RONS成分与浓度以及癌细胞灭活效率。当前的科学挑战是缺乏对RONS与pH协同抗癌效应和RONS生成途径的定量解析，这关系到能否实现精准优化灭活癌细胞。基于此，本项目先通过清除剂技术定量解析出酸/碱性等离子体活化液中不同RONS和pH协同灭活黑色素瘤的权重，找出起抗癌效应的RONS；然后采用清除剂与捕捉剂对RONS生成途径进行定量解析，阐释酸/碱环境下RONS生成途径差异性的内在机理；优化RONS浓度和灭活效率随放电参数的变化规律，针对酸/碱性活化液的抗癌效应提出多参数协同的优化调控策略。本项目的实施将有助于解决RONS生成途径的瓶颈问题，为等离子体治疗癌症的优化应用研究提供重要参考依据。

In recent years, atmospheric pressure cold plasma technology has shown a broad application prospect in cancer therapy. At present, studies on plasma inactivated cancer cells mainly consider the role of aqueous reactive oxygen nitrogen species (RONS), but ignore the contribution of solution pH in anticancer. PH seriously controls the formation pathways of RONS, ultimately it affects the composition and concentration of RONS as well as the inactivation efficiency of cancer cells. The current scientific challenge is the lack of quantitative analysis of synergistically anticancer effects of RONS and pH and the formation pathways of RONS, this is related to the ability if can it be fulfilled for achieving precise optimization inactivation of cancer cells. Based on this, first this project would quantitatively analyze the weight of synergistic inactivation of melanoma for different RONS and pH in acid/alkaline plasma activated liquid by scavenge agent technology, find out the RONS in anti-cancer effect; then use the scavenge agent and the capture agent to quantitative analyze the RONS generation formation pathways, and elucidate the intrinsic mechanism for difference of formation pathways of RONS in acid/alkaline environments; optimize the variation law of RONS concentration and inactivation efficiency with discharge parameters, and propose multi-parameter optimal regulation strategies in view of the anticancer effect of acid/alkaline plasma activated liquid. The implementation of this project will help to solve the bottleneck of the formation pathways of RONS, and provide an important reference for research of the optimization application of plasma in treatment of cancer.