液相活性粒子（如NO2-、NO3-、H2O2、OH、O2-、1O2等）在等离子体生物医学研究和应用中发挥着重要作用。现有诊断方法（酶标法和电子自旋共振法）只能对少数几种液相活性粒子的平均浓度进行定量诊断，但并不适用于活性粒子渗透深度的测量，而且需要在水溶液中添加特定的试剂（如显色剂、捕获剂等），这些试剂的加入会破坏原溶液系统的化学通路和反应动态平衡。基于此，本项目提出采用最新的光学诊断方法（吸收光谱拟合分离法和气-液界面激光全反射法），并与现有诊断方法相结合，开展对液相活性粒子定量诊断研究。获取活性粒子浓度随水溶液特性（去离子水、生理盐水和缓冲液）的变化规律，阐释不同水溶液中活性粒子浓度之间的内在关联机理。获取液相活性粒子浓度和渗透深度随外部参数的变化规律，提出一套多参数协同的优化调控方案。本项目的开展有助于突破现有诊断方法瓶颈，为液相活性粒子定量诊断提供一种切实可行的研究方案和技术支撑。

The aqueous reactive species (such as NO2-, NO3-, H2O2, OH, O2-, 1O2, etc) have an important role in the plasma biomedical research and application. The existing diagnosis methods (microplate reader and electron spin resonance spectrometer) can only quantitatively detect the average concentration of one of the few aqueous reactive species，but is not applicable to detect the penetration depth of aqueous reactive species, meanwhile, also need to add the specific reagent (such as chromogenic agent, capture agent, etc) in aqueous solution, the addition of these reagents would destroy the chemical pathways and reaction dynamic balance of system of the original solution. Based on them, this project propose to adopt the latest optical diagnosis methods (absorption spectrum fitting separation method and gas-liquid interface laser total reflection method), and combine with the existing diagnosis methods to carry out quantitative diagnostic studies of aqueous reactive species, obtain the change rule of the concentration of aqueous reactive species changing with the properties of aqueous solution (deionized water, normal saline, and buffer solution), illustrate the inner relation mechanism between concentrations of aqueous reactive species in different aqueous solutions. Obtain the change rule of the concentration and penetration depth of aqueous reactive species changing with external parameters, put forward a set of multi-parameter coordinated optimization control scheme. The implementation of this project would be helpful to break through the bottleneck of existing diagnosis methods, and provide a feasible research plan and technical support for quantitative diagnosis of aqueous reactive species.