基于比率电化学传感器阵列的农产品中真菌毒素检测研究

真菌毒素毒性强、种类多，农产品受多种毒素混合污染的情况较为常见，而毒素间协同效应会加剧被污染物的毒性。本研究项目聚焦农产品真菌毒素污染现状，发挥分析化学与农业工程学科交叉优势，拟开展可实现连续重复检测的比率电化学适配体传感器阵列研究，重点解决农产品中多种真菌毒素的同时精准快速检测难题。拟引入电化学辅助剥离技术，发展MXene纳米敏感材料的可控制备新方法，结合适配体组装与界面功能化技术，提出针对单一真菌毒素的高效敏感界面构筑策略；探讨比率法对敏感界面响应性能的作用规律，揭示比率传感器连续检测性能的工作机制，创制系列比率电化学适配体传感器件，突破适配体传感器无法连续重复检测瓶颈；结合丝网印刷与自动点样技术，发展比率电化学适配体传感阵列批量构筑与连续检测应用方法，解决多通道信号精准采集与处理难题，实现农产品中多种真菌毒素同时精准检测。本项目的顺利实施将有力推动电化学传感方法在农业领域的应用。

Various mycotoxins with relatively high toxicity can be always found in agricultural productions which endow these productions with more serious harm to human because of synergistic effect. Our project focuses on the monitoring of mycotoxin contamination of agricultural productions, and aims to develop ratiometric electrochemical aptasensor array to achieve the simultaneous and rapid detection of multiple mycotoxins with high accuracy. We would develop novel method for the controllable preparation of MXene based on electrochemical stripping technology, and then construct high-performance sensing interface via the assembling of aptamer and the surface functionalization. Subsequently, the influence of ratiometry on the properties of sensing interface as well as detection principle of ratiometric aptasensing would be comprehensively discussed, and then novel ratiometric electrochemical aptasensors are to be fabricated to achieve the continuous detection of the model target. At last, strategies for collection and data processing of multiple signals are to be developed, and then ratiometric electrochemical aptasensor array would be fabricated by combining screen printing and microarrayer to simultaneously and rapidly detect multiple mycotoxins in agricultural productions with high accuracy. Our investigation would promote the application of electrochemical aptasensing in agricultural industry.