新型纳米纤维自增强电化学发光生物传感器研究

为了增强电化学发光效率，简化实验体系，提高分析物的灵敏度和选择性，拟开展“新型纳米纤维自增强电化学发光生物传感器研究”。本项目拟选择合适的聚合物前体、具有特异性识别作用的生物分子、电化学发光分子及其共反应剂的混合物作为电纺溶液，优化电纺过程中各项参数，直接利用静电纺丝技术，无需碳化，一步简单制备一种导电性好、 灵敏度高且具有特异性识别作用的自增强电化学发光纳米纤维复合物。该材料将生物分子、电化学发光分子及其共反应剂同时负载到纳米纤维中，增强电化学发光信号，提高稳定性的同时，使纤维膜具备特异性识别作用。由于制备的材料具有自支撑的膜结构，因此可直接用作电极材料，实现对DNA（如甲基化DNA）、蛋白质(如凝血酶、癌胚抗原）等生物大分子的简单、灵敏及特异性的分析。

The project is focus on "The preparation of novel self-enhanced electrochemiluminescence nanofibers and their application in biosensors" to enhance the luminous efficiency, simplify the experiment system, improve the analysis sensitivity and selectivity. For this, we attempt to develop a simple method to synthesize a self-enhanced elecctrochemiluminescence nanofiber with good conductivity and specific recognition function by electrospinning the mixture of suitable polymer, biological molecule, electrochemiluminescence molecule and its co-reactant. Since biological molecule, electrochemiluminescence molecule and its co-reactant were loaded on the nanofiber at the same time, the stability was improved. Moreover, the prepared nanofiber can produce high electrochemiluminescence signal by itself and recognize the corresponding target biological molecule specifically. In addition, since the prepared nanofiber is self-standing structure, it can be used as electrode directly for the simply, sensitively and selectively analysis of DNA (e.g. DNA methylation), protein (e.g. thrombin, carcinoembryonic antigen) and so on.