甘蓝型油菜种子发育过程中含油量动态QTL检测及阶段性油脂积累关键候选基因鉴定

油菜种子发育不同阶段油脂合成速率不同，说明油脂代谢相关基因在不同发育阶段存在不同的表达和调控模式。目前，关于油菜含油量QTL定位均是在成熟种子中进行的。开展不同发育阶段种子动态含油量QTL分析、鉴定阶段特异和稳定的QTL是针对含油量研究的一种新思路，对阐明种子发育过程中油脂合成的基因表达调控机制具有重要意义。本项目拟通过甘蓝型油菜分离群体种子发育过程中各阶段含油量和脂肪酸组分连续动态监测，进行种子不同发育阶段含油量和脂肪酸组分的动态QTL分析，结合种子发育过程中多阶段BSR-Seq进行含油量关联位点鉴定和基因表达差异分析，鉴定导致高油和低油材料在不同阶段油脂积累速率差异的关键基因或转录因子，并通过对目标候选基因超表达和CRISPR/Cas9基因编辑技术进行功能分析，为解析油菜种子发育过程中油脂积累模式和进一步提高油菜种子含油量奠定基础。

The synthetic rate of lipid was different in different developmental stages, this mean that the genes that related to lipid metabolism were possibly with different expression and regulation pattern in different developmental stages. At present, the researches on QTL mapping for oil content were all in mature seeds in rapeseed. So, measure the oil content in different developmental stages of mapping population, perform the dynamic oil QTL analysis and identify the stage-specific and stage-stable QTL were a new research ideas for oil content. It was significant for make clear the mechanism of gene expression and regulation for lipid synthesis, which was also provided the new ideas for cultivation of new materials with high oil content. This project was to measure the dynamic oil content and fatty acid compositions in different developmental stages of segregation population and performed the dynamic QTL mapping, combining the results of muti-stages of BSR-Seq analysis, to identify the oil related key genes or transcript factors that caused the different lipid accumulation in high and low oil content materials. At last, over-expression and knock out the candidate genes to perform the function analysis. This research would lay a foundation for illustration of lipid accumulation pattern and to improve the oil content in rapeseed in the future.