提高油菜种子含油量是主要育种目标之一，传统关于含油量研究只是针对整粒种子进行，但油菜种子不同组织（种皮、外子叶、内子叶、胚轴等）的含油量存在较大差异，且这些差异产生的分子生物学基础还不清楚，这对进一步提高含油量造成了困难。对油菜种子不同部位油脂含量进行无损检测和三维成像分析并进行进行QTL解析，这对针对性提高油菜种子不同部位的含油量进而提高种子的总含油量十分重要。本项目拟利用基于核磁共振的非损伤性种子油脂含量检测技术和三维成像技术对定位群体种子各组织油脂分布和含油量进行分析和QTL定位，以揭示不同组织特异QTL位点和共有QTL位点，比较基因组分析QTL区间可能存在的基因并结合通过转录组获得的种子不同组织的差异表达基因，最终确定油菜种子各组织共有和组织特异的基因并进行基因功能分析。本研究将会为揭示油菜种子不同组织含油量形成分子生物学基础和进一步提高油菜种子含油量奠定基础。

Increase the oil content is the main research project in rapeseed breeding. In the traditional research, the research on the oil content was just focus on oil content in the whole seed. But the oil content in different tissues of rapeseed (seed coat, outer cotyledon, inner cotyledon and hypocotyl, etc.) was varied a lot, and the molecular basis of different tissues of seed remained unclear, which makes it difficult to increase oil content in future. So, genetic dissection of the accumulation mechanism of oil content in different tissues of rapeseed seeds is of great importance for increasing the total oil content by specifically increasing the oil content in different tissues. This project intends to utilize the non-invasive and 3D imaging method to analysis the seed oil content and the oil distribution in different tissues of rapeseed in DH population, and accurately identify the tissue-specific QTLs and commen QTLs. By using comparative genomics to identified the genes in QTL region combined with the differentally expressed genes that obtained through the transcriptomes of different tissues at different developmental stages, the tissue common and tissue specific genes would be selected and performed function analysis at last. The research would be laid the foundation for revealed the molecular biology basis of oil accumulation in different tissues of rapeseed and to increase the oil content of rapeseed seeds in future.