水稻条纹花叶病毒招募m6A修饰系统调控病毒侵染的分子机理

N6-甲基腺苷（N6-methyl-adenosine，m6A）是一种动态可逆的RNA甲基化修饰，近年来被证明在侵染哺乳动物的RNA病毒致病过程中发挥重要作用。在植物中，m6A修饰极有可能也是一种调控RNA病毒在宿主体内复制的机制。水稻条纹花叶病毒（Rice stripe mosaic virus，RSMV）是严重威胁我国水稻生产的细胞质弹状病毒新种，基因组为负义单链RNA。我们发现RSMV编码的糖蛋白G和水稻m6A甲基转移酶OsMTA4存在直接的互作，且证实了RSMV基因组存在m6A修饰。本项目拟通过分子生物学、生物化学和细胞生物学的手段解析RSMV编码G蛋白招募m6A甲基转移酶的互作机制，探究m6A修饰对RSMV基因组稳定性、蛋白表达、vsiRNA含量与分布的影响，最终阐明RSMV招募m6A修饰系统并调控病毒侵染的分子机理。研究结果将为了解RNA表观修饰在病毒侵染中的作用提供新的发现。

N6-methyl-adenosine (m6A) is a dynamic and reversible RNA methylation modification. In recent years, m6A has been proved to play an important role in the pathogenesis of mammalian RNA viruses. In plants, m6A modification may also be a mechanism to regulate RNA virus replication in host. Rice stripe mosaic virus (RSMV) is a newly discovered Cytorhabdovirus with negative single stranded RNA genome. We found a direct interaction between RSMV encoded glycoprotein G and rice m6A methyltransferase OsMTA4, and confirmed that there are m6A modifications in RSMV genome. In this project, we intend to investigate the interaction mechanism of RSMV encoded G protein recruiting m6A methyltransferase by using molecular biology, biochemistry and cell biology technologies, and to explore the effect of m6A modification on the stability of RSMV genome, protein expression, vsiRNA content and distribution. Finally, the effect of m6A modification in RSMV genome on virus infection and its mechanism will be elucidated. The results will provide new discovery and ideas for understanding the role of RNA epigenetic modification in virus infection.