近年来南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus，SRBSDV)对我国及周边国家的水稻生产造成了严重危害。我们前期研究发现，SRBSDV编码的P6蛋白通过激活水稻乙烯信号来提高对介体白背飞虱的引诱能力，从而促进病毒传播扩散，且通过免疫共沉淀发现P6能与水稻乙烯信号转导中的关键蛋白OsEBF1互作。本项目拟对SRBSDV P6效应蛋白-寄主因子的分子互作开展深入研究：通过分子生物学、生物化学、细胞生物学等研究手段剖析P6效应蛋白与寄主因子OsEBF1的互作机制，解析P6对水稻乙烯信号转导的调控机理；对互作因子OsEBF1的超表达和基因编辑突变体进行生物学验证，明确乙烯信号介导SRBSDV提高水稻引诱白背飞虱的机制，最终阐明SRBSDV调控水稻乙烯信号转导促进介体传毒的分子机理。研究结果将为防控SRBSDV的流行提供新的理论依据。

Over past few years, Southern rice black-streaked dwarf virus (SRBSDV) has caused serious loss in rice production. Our previous study found that the effector P6 of SRBSDV could activate the ethylene signaling of rice plants to increase the attractiveness to the vector white-backed planthopper, which facilitates transmission and spread of the virus, and a key protein in rice ethylene signal transduction, OsEBF1, was identified interacting with the effector P6 by immunoprecipitation. In this project, further studies via molecular biology, biochemistry, cell biology and other research methods will carried out to elucidate the interaction of virus effector and host factor, uncover the interaction mechanism of virus effector and host factor, resolve the regulation mechanism of P6 via OsEBF1 on ethylene signal transduction in rice, verify the biological phenomenon by generating overexpression and gene editing mutants of OsEBF1, and clarify the mechanism of ethylene pathway mediating SRBSDV improved vector attractiveness of rice plants. Finally, our results will elucidate the molecular mechanism of SRBSDV modulated ethylene signal transduction in rice to promote the virus transmission, and the results will provide a new theoretical basis for the prevention and control of the spread of SRBSDV.