雨生红球藻Haematococcus pluvialis WBG-26对壶菌感染的抗性机制研究

雨生红球藻是天然虾青素的最佳来源，然而雨生红球藻规模化养殖过程中经常发生壶菌感染，严重影响虾青素产量与品质，制约其产业的发展。由于对雨生红球藻抗壶菌感染机制知之甚少，雨生红球藻壶菌病害的防治一直未取得进展。本项目组在前期研究中筛选到一株对壶菌感染具有抗性的雨生红球藻WBG-26，其优异的抗感染特性在室外规模培养中得到了验证。以此为切入点，本项目拟以雨生红球藻抗性藻株和易感藻株为研究材料，利用细胞生物学、生理生化的方法研究二者在壶菌P. sedebokerense胁迫下的抗性差异；从雨生红球藻-壶菌互作视角，利用互作转录组测序和蛋白质组学技术，结合基因组重测序联合分析抗性和易感藻株的基因和蛋白质表达谱差异，挖掘抗性基因，阐明雨生红球藻对壶菌感染的抗性机制。研究结果将为利用现代技术高效选育雨生红球藻抗性藻株提供理论依据，加速抗性藻株的选育和病害防治技术的建立，促进我国红球藻产业的健康发展。

Mass cultivation of Haematococcus pluvialis is threatened by a destructive fungus Paraphysoderma sedebokerense, which can cause huge losses in astaxanthin production. However, the biochemical and molecular resistance mechanisms of H. pluvialis to P. sedebokerense remain virtually unknown, which restrict the prevention and control of H. pluvialis disease. In our previous study, the resistant strain of H. pluvialis was screened and its excellent anti-infective properties were verified in mass cultivation. In the present study, the resistant strain H. pluvialis WBG-26 and the high susceptible strain H. pluvialis K-0084 were selected as materials to analyze the response differences between them after infected with P. sedebokerense through cell biology and biochemical study. Moreover, dual RNA-Seq , iTRAQ and whole genome resequencing were selected to identify the genes and proteins related to disease resistance and explore the physiological and molecular resistance mechanism of H. pluvialis to P. sedebokerense. Through the combination of multi-omics data and conjoint analysis, the results of this study will provide a new idea for the screening of the resistance H. pluvialis strains to P. sedebokerense and have a wide application future.