十二烷基苯磺酸钠控制产油微藻真菌感染的作用机理及技术优化研究

产油微藻培养过程中的生物污染是制约微藻生物柴油产业化的关键技术难题之一，其中寄生真菌感染发生快、危害大，常导致培养失败，目前还没有开发出高效经济的控制技术。申请人前期研究中发现，阴离子表面活性剂十二烷基苯磺酸钠可以有效控制真菌感染而对产油微藻的生长和油脂积累不产生显著影响。十二烷基苯磺酸钠以前从未被用于控制微藻真菌感染，阐明其作用机理具有重要的科学价值。本项目以适合于开放池规模培养的产油微藻优良藻种油球藻（Graesiella sp. WBG-1）为材料，利用已建立的油球藻-寄生真菌（Amoeboaphelidium protococcarum）感染模型，探明十二烷基苯磺酸钠控制真菌感染的作用位点，研究其控制机理，优化影响控制效果的重要条件，建立高效、环保的十二烷基苯磺酸钠控制真菌感染的技术。研究结果将为解决微藻规模培养中真菌感染这一全球性的技术难题探索出一条全新的途径，应用前景广阔。

Oleaginous microalgae has been taken as one of significant potential feedstocks for biodiesel production. However, one problem in mass cultivation of microalgae is the frequent occurrence of infection with algal parasite, and for now no effective approaches are found to control the fungal parasite. In our previous study, we found out an anionic surfactant (sodium dodecyl benzene sulfonate) with high efficiency on fungal pests. In this project, on the basis of established oleaginous microalgae -fungal infection mode, we will elucidate the mechanism of surfactant against fungus from the target site of action and action mode; further improve the efficiency of the surfactant. Lastly, the high-efficiency and environmental surfactant control technology with best strategy will be established. The results of this study will provide a new pathway to solve this global technical fungal problem in mass cultivation of microalgae and have a wide application future.